ArticleOncogene2002
Interplay between cdk9 and NF-kappaB factors determines the level of HIV-1 gene transcription in astrocytic cells.
Article in Oncogene, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasis.eLife · 2021Article
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- Flavopiridol Protects Bone Tissue by Attenuating RANKL Induced Osteoclast Formation.Frontiers in pharmacology · 2018Article
- Non-Metabolic Role of PKM2 in Regulation of the HIV-1 LTR.Journal of cellular physiology · 2017Article
- Biochemical and cellular characterization of transcription factors binding to the hyperconserved core promoter-associated M4 motif.BMC genomics · 2016Article
- Article
- Deregulations in the cyclin-dependent kinase-9-related pathway in cancer: implications for drug discovery and development.ISRN oncology · 2013Article
- Growth inhibition of malignant glioblastoma by DING protein.Journal of neuro-oncology · 2012Article
- Transcriptional regulation of HIV-1 gene expression by p53.Cell cycle (Georgetown, Tex.) · 2010Article
- P-TEFb kinase complex phosphorylates histone H1 to regulate expression of cellular and HIV-1 genes.The Journal of biological chemistry · 2010Article
- CDKN1C negatively regulates RNA polymerase II C-terminal domain phosphorylation in an E2F1-dependent manner.The Journal of biological chemistry · 2010Article
- Specification of DNA binding activity of NF-kappaB proteins.Cold Spring Harbor perspectives in biology · 2009Review
- Evidence for activation of the TGF-beta1 promoter by C/EBPbeta and its modulation by Smads.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2009Article
- St. John's Wort protein, p27SJ, regulates the MCP-1 promoter.Molecular immunology · 2008Article
- Contribution of TRPV1 to microglia-derived IL-6 and NFkappaB translocation with elevated hydrostatic pressure.Investigative ophthalmology & visual science · 2008Article
- Evidence for BAG3 modulation of HIV-1 gene transcription.Journal of cellular physiology · 2007Article
- p73 Interacts with human immunodeficiency virus type 1 Tat in astrocytic cells and prevents its acetylation on lysine 28.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Basal transcription of the HIV-1 genome is controlled by a variety of ubiquitous and inducible regulatory factors, some with the ability to associate with the viral DNA sequences within the promoter spanning the long terminal repeat (LTR). In this report we demonstrate that activation of the HIV-1 promoter through the inducible DNA binding NF-kappaB transcription factors can be affected by cdk9 in human astrocytic cells. Our results show that ectopic expression of cdk9, but not its mutant variant which lacks the domain responsible for its kinase activity, augments transcription of the LTR. Moreover, we demonstrate that induction of the NF-kappaB pathway by PMA, or overexpression of its subunits including p50/p65 have a negative effect on the ability of cdk9 to stimulate viral gene transcription in these cells. Results from band-shift experiments demonstrated significant suppression of p50/p65 association to its DNA target motif by cdk9. Further, data from GST pull-down and combined immunoprecipitation/Western blot analysis of the protein extracts from cells expressing cdk9, p50 and p65 have revealed the interaction of cdk9 with both p50 and p65 in the absence of DNA containing the kappaB motif. All of these observations led us to conclude that the interaction of cdk9 with the NF-kappaB factors can determine the ability of NF-kappaB to modulate HIV-1 gene transcription.
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