ArticleThe Plant cell2002
Three novel MYB proteins with one DNA binding repeat mediate sugar and hormone regulation of alpha-amylase gene expression.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers, 1 of them a synthesis that pooled it.
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Who cites it
117 citing papers in PubMed, 1 synthesis or guideline pooled it, 268 citations in OpenAlex.
- Comprehensive meta-analysis, co-expression, and miRNA nested network analysis identifies gene candidates in citrus against Huanglongbing disease.BMC plant biology · 2015Pooled it
- Article
- Integrative transcriptomics nominates a 1R-MYB candidate associated with epidermal wax-related traits and osmotic-stress responses in Kentucky bluegrass.Frontiers in plant science · 2026Article
- cis- and trans-elements for the transcriptional regulation of sugar responsive genes: from current knowledge to future applications.Plant molecular biology · 2025Review
- Stripe rust fungi hijack a host transcriptional repressor to induce TaSWEET14d for enhanced sugar uptake in wheat.Science advances · 2025Article
- Identification of Submergence Tolerance Loci in Dongxiang Wild Rice (DXWR) by Genetic Linkage and Transcriptome Analyses.International journal of molecular sciences · 2025Article
- Research progress on the synergistic regulation of MYB transcription factor-mediated developmental plasticity and stress responses in rice.Frontiers in plant science · 2025Review
- Overexpression ofFrontiers in plant science · 2025Article
- Advances in seed hypoxia research.Plant physiology · 2024Article
- Genetic factors underlying anaerobic germination in rice: Genome-wide association study and transcriptomic analysis.The plant genome · 2024Article
- Recent progress in understanding the cellular and genetic basis of plant responses to low oxygen holds promise for developing flood-resilient crops.Journal of experimental botany · 2024Article
- High-yield BMP2 expression in rice cells via CRISPR and endogenous αAmy3 promoter.Applied microbiology and biotechnology · 2024Article
- Article
- Integrated transcriptomic and proteomic analysis of exogenous abscisic acid regulation on tuberous root development inFrontiers in nutrition · 2024Article
- AtMYBS1 negatively regulates heat tolerance by directly repressing the expression of MAX1 required for strigolactone biosynthesis in Arabidopsis.Plant communications · 2023Article
- CIPK15-mediated inhibition of NHHeliyon · 2023Article
- SnRK1 kinase-mediated phosphorylation of transcription factor bZIP39 regulates sorbitol metabolism in apple.Plant physiology · 2023Article
- Transcriptome Analysis of Rice Embryo and Endosperm during Seed Germination.International journal of molecular sciences · 2023Article
- Cytological, transcriptome and miRNome temporal landscapes decode enhancement of rice grain size.BMC biology · 2023Article
- The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (Plants (Basel, Switzerland) · 2023Article
57 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of alpha-amylase genes in cereals is induced by both gibberellin (GA) and sugar starvation. All alpha-amylase genes isolated from cereals contain a TATCCA element or its variants at positions approximately 90 to 150 bp upstream of the transcription start sites. The TATCCA element was shown previously to be an important component of the GA response complex and the sugar response complex of alpha-amylase gene promoters. In the present study, three cDNA clones encoding novel MYB proteins with single DNA binding domains were isolated from a rice suspension cell cDNA library and designated OsMYBS1, OsMYBS2, and OsMYBS3. Gel mobility shift experiments with OsMYBSs showed that they bind specifically to the TATCCA element in vitro. Yeast one-hybrid experiments demonstrated that OsMYBS1 and OsMYBS2 bind to the TATCCA element and transactivate a promoter containing the TATCCA element in vivo. Transient expression assays with barley half-seeds showed that OsMYBS1 and OsMYBS2 transactivate a promoter containing the TATCCA element when sugar is provided, whereas OsMYBS3 represses transcription of the same promoter under sugar starvation. Transient expression assays also showed that these three OsMYBSs cooperate with a GA-regulated transcription factor, HvMYBGa, in the transactivation of a low-pI barley alpha-amylase gene promoter in the absence of GA. Two-hybrid experiments with barley half-seeds showed that OsMYBS1 is able to form a homodimer. The present study demonstrates that differential DNA binding affinity, promoter transactivation ability, dimerization, and interactions with other protein factors determine the biological function of OsMYBSs. This study also suggests that common transcription factors are involved in the sugar and hormonal regulation of alpha-amylase gene expression in cereals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.