ArticleThe Plant cell2002
The POLARIS gene of Arabidopsis encodes a predicted peptide required for correct root growth and leaf vascular patterning.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 199 citations in OpenAlex.
- Plant Peptides on the Rise: From Historical Insight to Future Applications.Plant biotechnology journal · 2026Review
- Computational identification and characterization of noncoding RNA-encoded peptides: tools, databases, and in silico strategies.Amino acids · 2026Review
- Plant non-canonical peptides: From identification to mechanisms.Plant communications · 2026Review
- POLARIS is a copper-binding peptide that interacts with ETR1 to negatively regulate ethylene signaling in Arabidopsis.Plant communications · 2025Article
- Peptide Hormone-Mediated Regulation of Plant Development and Environmental Adaptability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Review
- MDF Regulates a Network of Auxin-Dependent and Auxin-Independent Pathways of Adventitious Root Regeneration inPlant direct · 2025Article
- Peptide hormones in plants.Molecular horticulture · 2025Review
- Article
- A predictive model for ethylene-mediated auxin and cytokinin patterning in the Arabidopsis root.Plant communications · 2024Article
- Biological Activity of Artificial Plant Peptides Corresponding to the Translational Products of Small ORFs in Primary miRNAs and Other Long "Non-Coding" RNAs.Plants (Basel, Switzerland) · 2024Review
- Review
- Two neuronal peptides encoded from a single transcript regulate mitochondrial complex III ineLife · 2022Article
- Small open reading frames in plant research: from prediction to functional characterization.3 Biotech · 2022Review
- Pervasive translation of small open reading frames in plant long non-coding RNAs.Frontiers in plant science · 2022Review
- Large-scale discovery of non-conventional peptides in grape (Horticulture research · 2022Article
- Peptidome: Chaos or Inevitability.International journal of molecular sciences · 2021Review
- A New Type of Satellite Associated with Cassava Mosaic Begomoviruses.Journal of virology · 2021Article
- Root growth responses to mechanical impedance are regulated by a network of ROS, ethylene and auxin signalling in Arabidopsis.The New phytologist · 2021Article
- PIP2, An Auxin Induced Plant Peptide Hormone Regulates Root and Hypocotyl Elongation inFrontiers in plant science · 2021Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The POLARIS (PLS) gene of Arabidopsis was identified as a promoter trap transgenic line, showing beta-glucuronidase fusion gene expression predominantly in the embryonic and seedling root, with low expression in aerial parts. Cloning of the PLS locus revealed that the promoter trap T-DNA had inserted into a short open reading frame (ORF). Rapid amplification of cDNA ends PCR, RNA gel blot analysis, and RNase protection assays showed that the PLS ORF is located within a short ( approximately 500 nucleotides) auxin-inducible transcript and encodes a predicted polypeptide of 36 amino acid residues. pls mutants exhibit a short-root phenotype and reduced vascularization of leaves. pls roots are hyperresponsive to exogenous cytokinins and show increased expression of the cytokinin-inducible gene ARR5/IBC6 compared with the wild type. pls seedlings also are less responsive to the growth-inhibitory effects of exogenous auxin and show reduced expression of the auxin-inducible gene IAA1 compared with the wild type. The PLS peptide-encoding region of the cDNA partially complements the pls mutation and requires the PLS ORF ATG for activity, demonstrating the functionality of the peptide-encoding ORF. Ectopic expression of the PLS ORF reduces root growth inhibition by exogenous cytokinins and increases leaf vascularization. We propose that PLS is required for correct auxin-cytokinin homeostasis to modulate root growth and leaf vascular patterning.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.