ArticleGut2002
Modulation of host antimicrobial peptide (beta-defensins 1 and 2) expression during gastritis.
Article in Gut, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 99 citations in OpenAlex.
- Host Cell Antimicrobial Responses againstInternational journal of molecular sciences · 2022Review
- The Challenges of Eradicating PediatricChildren (Basel, Switzerland) · 2022Review
- Gastric alarmin release: A warning signal in the development of gastric mucosal diseases.Frontiers in immunology · 2022Review
- Review
- Effects ofInnate immunity · 2021Article
- DEFB4A is a potential prognostic biomarker for colorectal cancer.Oncology letters · 2020Article
- A Novel View of HumanBiomolecules · 2019Review
- Beta-defensins and analogs in Helicobacter pylori infections: mRNA expression levels, DNA methylation, and antibacterial activity.PloS one · 2019Article
- β-Defensins in the Fight against Helicobacter pylori.Molecules (Basel, Switzerland) · 2017Review
- IL-22-induced antimicrobial peptides are key determinants of mucosal vaccine-induced protection against H. pylori in mice.Mucosal immunology · 2017Article
- Clinical chorioamnionitis at term II: the intra-amniotic inflammatory response.Journal of perinatal medicine · 2016Article
- Reactive oxygen species, apoptosis, antimicrobial peptides and human inflammatory diseases.Pharmaceuticals (Basel, Switzerland) · 2015Review
- Host Antimicrobial Peptides in Bacterial Homeostasis and Pathogenesis of Disease.Antibiotics (Basel, Switzerland) · 2014Article
- Chemokines and antimicrobial peptides have a cag-dependent early response to Helicobacter pylori infection in primary human gastric epithelial cells.Infection and immunity · 2014Article
- Beta-Defensin 124 Is Required for Efficient Innate Immune Responses in Prostate Epithelial RWPE-1 Cells.Korean journal of urology · 2014Article
- Exploring alternative treatments for Helicobacter pylori infection.World journal of gastroenterology · 2014Review
- Helicobacter pylori downregulates expression of human β-defensin 1 in the gastric mucosa in a type IV secretion-dependent fashion.Cellular microbiology · 2013Article
- Gastric antimicrobial peptides fail to eradicate Helicobacter pylori infection due to selective induction and resistance.PloS one · 2013Article
- DEFB1 gene 5' untranslated region (UTR) polymorphisms in inflammatory bowel diseases.Clinics (Sao Paulo, Brazil) · 2012Article
- Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundbeta-Defensins are a newly identified family of antimicrobial peptides that are expressed by epithelia on mucosal surfaces where their production is augmented by infection or inflammation. Helicobacter pylori colonises the gastric epithelium causing persistent gastric inflammation leading to antral and corpus gastritis, and peptic ulcer disease.
aimsTo evaluate the role of beta-defensins in the innate immune response of the gastric epithelium to infection and inflammation, we have assessed mRNA expression and regulation of human beta-defensins 1 and 2 (hBD1, hBD2) by H pylori and proinflammatory stimuli. We have also compared gene and peptide expression of these bactericidal agents in H pylori induced gastritis with that in normal gastric mucosa.
methodsModulation of expression of hBD1 and hBD2 by various stimuli was studied in three (AGS, MKN7, MKN45) gastric epithelial cell lines by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). Defensin mRNA expression was measured by semiquantitative RT-PCR in gastritis tissue and compared with controls. Peptide localisation was assessed by immunohistochemistry.
resultsCytotoxic H pylori and interleukin 1 beta (IL-1 beta) markedly upregulated expression of hBD2 in a dose and time dependent manner in both AGS and MKN7 cell lines. A modest increase in hBD1 expression was also noted during infection. Interestingly, induction of hBD1 gene expression by IL-1 beta was only observed in MKN7 cells. The magnitude of this response was delayed and reduced compared with hBD2 expression. In gastric biopsies, hBD2 was undetectable in normal gastric antrum but a marked increase was observed in H pylori positive gastritis compared with control tissue (p<0.001). Constitutive expression of hBD1 was observed in normal gastric mucosa and there was a significant increase in gastritis (p<0.05). Immunohistochemistry revealed a parallel increase in hBD1 and hBD2 peptide expression in gastritis tissue with positive staining confined to the surface epithelium of the gastric glands.
conclusionsModulation of beta-defensin expression by pathogenic and/or inflammatory stimuli and their cellular localisation places these antimicrobial peptides in the front line of innate host defence in the human stomach.
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