ArticleThe EMBO journal2002
IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Architecture of the Tn7 posttransposition complex: an elaborate nucleoprotein structure.Journal of molecular biology · 2010Article
- The dynamic Mu transpososome: MuB activation prevents disintegration.Journal of molecular biology · 2007Article
- Control of transposase activity within a transpososome by the configuration of the flanking DNA segment of the transposon.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The positive and negative regulation of Tn10 transposition by IHF is mediated by structurally asymmetric transposon arms.Nucleic acids research · 2003Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The frequency of DNA transposition in transposition systems that employ a strand transfer step may be significantly affected by the occurrence of a disintegration reaction, a reaction that reverses the strand transfer event. We have asked whether disintegration occurs in the Tn10 transposition system. We show that disintegration substrates (substrates constituting one half of the strand transfer product) are assembled into a transpososome that mimics the strand transfer intermediate. This strand transfer transpososome (STT) does appear to support an intermolecular disintegration reaction, but only at a very low level. Strikingly, assembly of the STT is not dependent on IHF, a host protein that is required for de novo assembly of all previously characterized Tn10 transpososomes. We suggest that disintegration substrates are able to form both transposon end and target type contacts with transposase because of their enhanced conformational flexibility. This probably allows the conformation of DNA within the complex that prevents the destructive disintegration reaction, and is responsible for relaxing the DNA sequence requirements for STT formation relative to other Tn10 transpososomes.
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Registered trials
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