ArticleJournal of bacteriology2002
Growth phase-dependent regulation of target gene promoters for binding of the essential orphan response regulator HP1043 of Helicobacter pylori.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 59 citations in OpenAlex.
- Novel Drug-like HsrA Inhibitors Exhibit Potent Narrow-Spectrum Antimicrobial Activities againstInternational journal of molecular sciences · 2024Article
- Insights into the Orchestration of Gene Transcription Regulators inInternational journal of molecular sciences · 2022Review
- Two-component regulatory systems inFrontiers in cellular and infection microbiology · 2022Review
- 1,4-Dihydropyridine as a Promising Scaffold for Novel Antimicrobials AgainstFrontiers in microbiology · 2022Article
- Definition of the Binding Architecture to a Target Promoter of HP1043, the Essential Master Regulator ofInternational journal of molecular sciences · 2021Article
- HP1021 is a redox switch protein identified in Helicobacter pylori.Nucleic acids research · 2021Article
- Repurposing Dihydropyridines for Treatment ofPharmaceutics · 2019Article
- Identifying potential novel drugs against Helicobacter pylori by targeting the essential response regulator HsrA.Scientific reports · 2019Article
- TheFrontiers in microbiology · 2018Article
- Insight into the essential role of the Helicobacter pylori HP1043 orphan response regulator: genome-wide identification and characterization of the DNA-binding sites.Scientific reports · 2017Article
- The cag-pathogenicity island encoded CncR1 sRNA oppositely modulates Helicobacter pylori motility and adhesion to host cells.Cellular and molecular life sciences : CMLS · 2016Article
- Expansion of the tetracycline-dependent regulation toolbox for Helicobacter pylori.Applied and environmental microbiology · 2015Article
- Response to metronidazole and oxidative stress is mediated through homeostatic regulator HsrA (HP1043) in Helicobacter pylori.Journal of bacteriology · 2014Article
- A variable homopolymeric G-repeat defines small RNA-mediated posttranscriptional regulation of a chemotaxis receptor in Helicobacter pylori.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Atypical response regulator ChxR from Chlamydia trachomatis is structurally poised for DNA binding.PloS one · 2014Article
- Mutations to essential orphan response regulator HP1043 of Helicobacter pylori result in growth-stage regulatory defects.Infection and immunity · 2013Article
- Backbone dynamics of an atypical orphan response regulator protein, Helicobacter pylori 1043.Molecules and cells · 2013Article
- Transcriptional regulation of the CmeABC multidrug efflux pump and the KatA catalase by CosR in Campylobacter jejuni.Journal of bacteriology · 2012Article
- Role of the Helicobacter pylori sensor kinase ArsS in protein trafficking and acid acclimation.Journal of bacteriology · 2012Article
- A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen Helicobacter pylori.Applied and environmental microbiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helicobacter pylori encodes three two-component systems and two orphan response regulators (RRs) that are predicted to be involved in transcriptional regulation. The HP1043 gene encodes an essential OmpR-like RR, 1043RR, for which no histidine kinase has been identified. Gel filtration and cross-linking experiments on the purified 1043RR protein reveals that this protein is a dimer and in vivo dimerization assays localize the dimerization to the N-terminal regulatory domain. DNA-binding studies have revealed two targets for specific binding of the 1043RR protein and moreover, phosphorylation of the protein was not needed for the activation of binding. Footprinting analysis demonstrated that the 1043RR protein binds to its own promoter, P(1043), overlapping the -35 promoter element from positions -17 to -45, suggesting that this protein is autoregulatory. In addition, it binds at a similar location, spanning nucleotides from positions -22 to -51 at the promoter of the methyl-accepting chemotaxis tlpB gene, P(tlpB). A possible inverted repeat was identified in the binding sites of both promoters. In an attempt to overexpress 1043RR in H. pylori, the 10-fold induction in transcription of a second copy of HP1043 with use of an inducible promoter failed to increase cellular levels of the RR protein, suggesting that 1043RR is tightly regulated at a posttranscriptional level. The P(1043) and P(tlpB) promoters were demonstrated to be coordinately regulated in response to growth phase in H. pylori. The essential role of HP1043 in encoding a cell cycle regulator is discussed.
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