ArticleMolecular and cellular biology2002
GAGA factor and the TFIID complex collaborate in generating an open chromatin structure at the Drosophila melanogaster hsp26 promoter.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 57 citations in OpenAlex.
- Transgenic tools for targeted chromosome rearrangements allow construction of balancer chromosomes in non-melanogaster Drosophila species.G3 (Bethesda, Md.) · 2022Article
- GAGA factor: a multifunctional pioneering chromatin protein.Cellular and molecular life sciences : CMLS · 2021Review
- The zinc-finger protein CLAMP promotesJournal of cell science · 2019Article
- Factor cooperation for chromosome discrimination in Drosophila.Nucleic acids research · 2019Article
- The GAGA factor regulatory network: Identification of GAGA factor associated proteins.PloS one · 2017Article
- New insights for Drosophila GAGA factor in larvae.Royal Society open science · 2015Article
- Article
- Enhancer RNA facilitates NELF release from immediate early genes.Molecular cell · 2014Article
- Mechanisms and proteins involved in long-distance interactions.Frontiers in genetics · 2014Review
- Widespread evidence of cooperative DNA binding by transcription factors in Drosophila development.Nucleic acids research · 2013Article
- A global change in RNA polymerase II pausing during the Drosophila midblastula transition.eLife · 2013Article
- Conserved boundary elements from the Hox complex of mosquito, Anopheles gambiae.Nucleic acids research · 2013Article
- Computational identification of diverse mechanisms underlying transcription factor-DNA occupancy.PLoS genetics · 2013Article
- Promoter-proximal pausing of RNA polymerase II: emerging roles in metazoans.Nature reviews. Genetics · 2012Review
- Alanine zipper-like coiled-coil domains are necessary for homotypic dimerization of plant GAGA-factors in the nucleus and nucleolus.PloS one · 2011Article
- Drosophila heat shock system as a general model to investigate transcriptional regulation.Cold Spring Harbor symposia on quantitative biology · 2010Review
- Contrasting patterns of transposable element insertions in Drosophila heat-shock promoters.PloS one · 2009Article
- Nuclear receptor SHP activates miR-206 expression via a cascade dual inhibitory mechanism.PloS one · 2009Article
- NELF-mediated stalling of Pol II can enhance gene expression by blocking promoter-proximal nucleosome assembly.Genes & development · 2008Article
- Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The upstream regulatory region of the Drosophila melanogaster hsp26 gene includes two DNase I-hypersensitive sites (DH sites) that encompass the critical heat shock elements. This chromatin structure is required for heat shock-inducible expression and depends on two (CT)n*(GA)n elements bound by GAGA factor. To determine whether GAGA factor alone is sufficient to drive formation of the DH sites, we have created flies with an hsp26/lacZ transgene wherein the entire DNA segment known to interact with the TFIID complex has been replaced by a random sequence. The replacement results in a loss of heat shock-inducible hsp26 expression and drastically diminishes nuclease accessibility in the chromatin of the regulatory region. Chromatin immunoprecipitation experiments show that the decrease in TFIID binding does not reduce GAGA factor binding. In contrast, the loss of GAGA factor binding resulting from (CT)n mutations decreases TFIID binding. These data suggest that both GAGA factor and TFIID are necessary for formation of the appropriate chromatin structure at the hsp26 promoter and predict a regulatory mechanism in which GAGA factor binding precedes and contributes to the recruitment of TFIID.
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