ArticleJournal of assisted reproduction and genetics2002
Analysis of human spermatozoa by fluorescence in situ hybridization with preservation of the head morphology is possible by avoiding a decondensation treatment.
Article in Journal of assisted reproduction and genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors at 1 institution in 1 country.
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Abstract
purposeDevelopment of a hybridization technique for spermatozoa allowing the preservation of the head morphology.
methodsFISH analysis of fixed semen samples from oligoasthenoteratozoospermia (OAT) patients with a normal somatic karyotype attending the Cytogenetics and the IVF Laboratories of a University hospital for semen analysis. In situ hybridization with centromeric probes for chromosomes X, Y, and 18 and locus specific probe for chromosome 21.
resultsMore than 95% of the sperm heads showed clear hybridization signals and a conserved morphology including the visualization of the tail. Few cells with splitted signals were not considered.
conclusionsThis is the first description of a simple and fast hybridization protocol for spermatozoa without a decondensation step, allowing preservation of the morphology of the sperm head that is particularly useful to correlate abnormal spermatozoa with specific chromosome aneuploidies. With this technique we were able to avoid troubles in interpretation of FISH spots that does not depend on the quality of nuclear decondensation, as it is the case in the previously described methods. Our goal was to demonstrate the efficiency of the method without loosing sperm head morphology. Further studies are needed to correlate the aneuploidy rates for specific chromosomes with sperm morphology.
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