ArticleProceedings of the National Academy of Sciences of the United States of America2002
Are Pfiesteria species toxicogenic? Evidence against production of ichthyotoxins by Pfiesteria shumwayae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 51 citations in OpenAlex.
- Review of Harmful Algal Blooms (HABs) Causing Marine Fish Kills: Toxicity and Mitigation.Plants (Basel, Switzerland) · 2023Review
- Great diversity of KSFEMS microbes · 2022Article
- Review
- Lack of evidence for contact sensitization by Pfiesteria extract.Environmental health perspectives · 2007Article
- Phenotypically different microalgal morphospecies with identical ribosomal DNA: a case of rapid adaptive evolution?Microbial ecology · 2007Article
- Occupational exposure to pfiesteria species in estuarine waters is not a risk factor for illness.Environmental health perspectives · 2006Article
- Development of a cob-18S rRNA gene real-time PCR assay for quantifying Pfiesteria shumwayae in the natural environment.Applied and environmental microbiology · 2005Article
- Demonstration of toxicity to fish and to mammalian cells by Pfiesteria species: comparison of assay methods and strains.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Characterization of ichthyocidal activity of Pfiesteria piscicida: dependence on the dinospore cell density.Applied and environmental microbiology · 2005Article
- Dimethylsulfoniopropionate metabolism by Pfiesteria-associated Roseobacter spp.Applied and environmental microbiology · 2004Article
- Mitogen-activated protein kinase in Pfiesteria piscicida and its growth rate-related expression.Applied and environmental microbiology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
The estuarine genus Pfiesteria has received considerable attention since it was first identified and proposed to be the causative agent of fish kills along the mid-Atlantic coast in 1992. The presumption has been that the mechanism of fish death is by release of one or more toxins by the dinoflagellate. In this report, we challenge the notion that Pfiesteria species produce ichthyotoxins. Specifically, we show that (i) simple centrifugation, with and without ultrasonication, is sufficient to "detoxify" water of actively fish-killing cultures of Pfiesteria shumwayae, (ii) organic extracts of lyophilized cultures are not toxic to fish, (iii) degenerate primers that amplify PKS genes from several polyketide-producing dinoflagellates failed to yield a product with P. shumwayae DNA or cDNA, and (iv) degenerate primers for NRPS genes failed to amplify any NRPS genes but (unexpectedly) yielded a band (among several) that corresponded to known or putative PKSs and fatty acid synthases. We conclude that P. shumwayae is able to kill fish by means other than releasing a toxin into bulk water. Alternative explanations of the effects attributed to Pfiesteria are suggested.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.