ArticleJournal of virology2002
Role of an essential triloop hairpin and flanking structures in the 3' untranslated region of Alfalfa mosaic virus RNA in in vitro transcription.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Positive strand RNA viruses differ in the constraints they place on the folding of their negative strand.RNA (New York, N.Y.) · 2022Article
- Impact of the Potential mViruses · 2022Article
- Thermodynamic characterization of RNA triloops.Biochemistry · 2010Article
- In vitro and in vivo studies of the RNA conformational switch in Alfalfa mosaic virus.Journal of virology · 2010Article
- A comparative analysis of the triloops in all high-resolution RNA structures reveals sequence structure relationships.RNA (New York, N.Y.) · 2007Article
- Long-distance RNA-RNA interactions between terminal elements and the same subset of internal elements on the potato virus X genome mediate minus- and plus-strand RNA synthesis.RNA (New York, N.Y.) · 2007Article
- A 5' RNA element promotes dengue virus RNA synthesis on a circular genome.Genes & development · 2006Article
- Similarities and differences between the subgenomic and minus-strand promoters of an RNA plant virus.Journal of virology · 2004Article
- The 5' untranslated region of alfalfa mosaic virus RNA 1 is involved in negative-strand RNA synthesis.Journal of virology · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The minus-strand promoter of Alfalfa mosaic virus (AMV), a tripartite plant virus belonging to the family Bromoviridae, is located within the 3'-terminal 145 nucleotides (nt), which can adopt a tRNA-like structure (TLS). This contrasts with the subgenomic promoter for RNA4 synthesis, which requires approximately 40 nt and forms a single triloop hairpin. Detailed analysis of the minus-strand promoter now shows that a similar triloop hairpin, hairpin E (hpE), is crucial for minus-strand synthesis. The loop sequence of hpE appeared to not be essential for RNA synthesis, whereas the identity and base-pairing capability of bases below the triloop were indeed essential. Reducing the size of the bulge loop of hpE triggered transcription from an internal site similar to the process of subgenomic transcription. Similar effects were observed when deleting (part of) the TLS, suggesting that tertiary contacts between hpE and the TLS prevent internal initiation. The data indicate that the minus-strand promoter hpE and the subgenomic promoter hairpin are equivalent in binding the viral polymerase. We propose that the major role of the TLS is to enforce the initiation of transcription by polymerase at the very 3' end of the genome.
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