ArticleJournal of virology2002
Retrovirus-associated heparan sulfate mediates immobilization and gene transfer on recombinant fibronectin.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Virus stamping for targeted single-cell infection in vitro and in vivo.Nature biotechnology · 2018Article
- Shedding of clinical-grade lentiviral vectors is not detected in a gene therapy setting.Gene therapy · 2015Article
- Tunable delivery of siRNA from a biodegradable scaffold to promote angiogenesis in vivo.Advanced materials (Deerfield Beach, Fla.) · 2014Article
- Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Hematopoietic stem cell gene therapy:assessing the relevance of preclinical models.Seminars in hematology · 2013Review
- Fibrin-mediated lentivirus gene transfer: implications for lentivirus microarrays.Journal of controlled release : official journal of the Controlled Release Society · 2010Article
- Entry kinetics and cell-cell transmission of surface-bound retroviral vector particles.The journal of gene medicine · 2010Article
- The effects of Runx2 immobilization on poly (epsilon-caprolactone) on osteoblast differentiation of bone marrow stromal cells in vitro.Biomaterials · 2010Article
- Matrix fibronectin binds gammaretrovirus and assists in entry: new light on viral infections.Journal of virology · 2007Article
- Primary attachment of murine leukaemia virus vector mediated by particle-associated heparan sulfate proteoglycan.The Biochemical journal · 2006Article
- Caveola-dependent endocytic entry of amphotropic murine leukemia virus.Journal of virology · 2005Article
- Gene delivery through cell culture substrate adsorbed DNA complexes.Biotechnology and bioengineering · 2005Article
- Human immunodeficiency virus type 1-derived lentivirus vectors pseudotyped with envelope glycoproteins derived from Ross River virus and Semliki Forest virus.Journal of virology · 2004Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant retroviruses have been shown to bind to fibronectin (FN) and increase the efficiency of gene transfer to a variety of cell types. Despite recent work to optimize gene transfer on recombinant FN, the mechanism of retrovirus binding to FN and the interactions of target cells with the bound virus remain elusive. We investigated the roles of virus surface glycoprotein (gp70), cell-conditioned medium, and proteoglycans in mediating retrovirus binding to FN. We also examined the role of Polybrene (PB) in these interactions. We found that gp70 is not involved in retrovirus binding to FN. Immobilization of the virus, however, does not overcome its receptor requirement, and gp70 is still needed for successful gene transfer. Our results clearly show that retrovirus binds FN through virus-associated heparan sulfate (HS) and that binding is necessary for transduction without PB. Two distinct modes of gene transfer occur depending on PB: (i) in the presence of PB, retrovirus interacts directly with the target cells; and (ii) in the absence of PB, retrovirus binds to FN and target cells interact with the immobilized virus. PB may promote the former mode by interacting with the virus HS and reducing the negative charge of the viral particles. Interestingly, the latter mode is more efficient, leading to significantly enhanced gene transfer. A better understanding of these interactions may provide insight into virus-cell interactions and lead to a more rational design of transduction protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.