ArticleThe American journal of pathology2002
Multicolor-FICTION: expanding the possibilities of combined morphologic, immunophenotypic, and genetic single cell analyses.
Article in The American journal of pathology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
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- Molecular characteristics of mantle cell lymphoma presenting with clonal plasma cell component.The American journal of surgical pathology · 2011Article
- Combined detection of Her2/neu gene amplification and protein overexpression in effusions from patients with breast and ovarian cancer.Journal of cancer research and clinical oncology · 2010Article
- Leukemia-associated genetic aberrations in mesenchymal stem cells of children with acute lymphoblastic leukemia.Journal of molecular medicine (Berlin, Germany) · 2010Article
- TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma.The Journal of experimental medicine · 2009Article
- Successful application of a direct detection slide-based sequential phenotype/genotype assay using archived bone marrow smears and paraffin embedded tissue sections.The Journal of molecular diagnostics : JMD · 2007Article
- Aberrant immunoglobulin class switch recombination and switch translocations in activated B cell-like diffuse large B cell lymphoma.The Journal of experimental medicine · 2007Article
- Telomeric IGH losses detectable by fluorescence in situ hybridization in chronic lymphocytic leukemia reflect somatic VH recombination events.The Journal of molecular diagnostics : JMD · 2007Article
- Fluorescence in situ hybridization analysis of immunoglobulin heavy chain translocations in plasma cell myeloma using intact paraffin sections and simultaneous CD138 immunofluorescence.The Journal of molecular diagnostics : JMD · 2006Article
- FISH analysis for the detection of lymphoma-associated chromosomal abnormalities in routine paraffin-embedded tissue.The Journal of molecular diagnostics : JMD · 2006Review
- Cyclin D1-negative mantle cell lymphoma: a clinicopathologic study based on gene expression profiling.Blood · 2005Article
- Molecular cytogenetic analyses of immunoglobulin loci in nodular lymphocyte predominant Hodgkin's lymphoma reveal a recurrent IGH-BCL6 juxtaposition.The Journal of molecular diagnostics : JMD · 2005Article
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- Molecular cytogenetic detection of chromosomal breakpoints in T-cell receptor gene loci.Leukemia · 2003Article
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8 authors.
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Abstract
Phenotypic and genotypic analyses of cells are increasingly essential for understanding pathogenetic mechanisms as well as for diagnosing and classifying malignancies and other diseases. We report a novel multicolor approach based on the FICTION (fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasms) technique, which enables the simultaneous detection of morphological, immunophenotypic, and genetic characteristics of single cells. As prerequisite, multicolor interphase fluorescence in situ hybridization assays for B-cell non-Hodgkin's lymphoma and anaplastic large-cell lymphoma have been developed. These assays allow the simultaneous detection of the most frequent primary chromosomal aberrations in these neoplasms, such as t(8;14), t(11;14), t(14;18), and t(3;14), and the various rearrangements of the ALK gene, respectively. To establish the multicolor FICTION technique, these assays were combined with the immunophenotypic detection of lineage- or tumor-specific antigens, namely CD20 and ALK, respectively. For evaluation of multicolor FICTION experiments, image acquisition was performed by automatic sequential capturing of multiple focal planes. Thus, three-dimensional information was obtained. The multicolor FICTION assays were applied to well-characterized lymphoma samples, proving the performance, validity, and diagnostic power of the technique. Future multicolor FICTION applications include the detection of preneoplastic lesions, early stage and minimal residual diseases, or micrometastases.
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