ArticleThe Biochemical journal2002
Denaturing action of urea and guanidine hydrochloride towards two thermophilic esterases.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
- Buttressing ligand-binding pockets in de novo designed NTF2-like domains.Protein science : a publication of the Protein Society · 2025Article
- The role of Gln269Leu mutation on the thermostability and structure of uricase from Aspergillus flavus.Scientific reports · 2025Article
- The pre-induction temperature affects recombinant HuGM-CSF aggregation in thermoinducible Escherichia coli.Applied microbiology and biotechnology · 2022Article
- General Counteraction Exerted by Sugars against Denaturants.Life (Basel, Switzerland) · 2021Article
- Development of an automated multienzymatic biosensor for risk assessment of pesticide contamination in water and food.EFSA journal. European Food Safety Authority · 2018Article
- Stabilization of an α/β-Hydrolase by Introducing Proline Residues: Salicylic Acid Binding Protein 2 from Tobacco.Biochemistry · 2015Article
- Fluorescence spectroscopy approaches for the development of a real-time organophosphate detection system using an enzymatic sensor.Sensors (Basel, Switzerland) · 2015Article
- Probing conformational stability and dynamics of erythroid and nonerythroid spectrin: effects of urea and guanidine hydrochloride.PloS one · 2015Article
- Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold.PloS one · 2015Article
- Structural changes in halophilic and non-halophilic proteases in response to chaotropic reagents.The protein journal · 2014Article
- Biophysical characterization of a recombinant aminopeptidase II from the thermophilic bacterium Bacillus stearothermophilus.Journal of biological physics · 2014Article
- pH-sensitive self-associations of the N-terminal domain of NBCe1-A suggest a compact conformation under acidic intracellular conditions.Protein and peptide letters · 2012Article
- Biophysical studies of an NAD(P)(+)-dependent aldehyde dehydrogenase from Bacillus licheniformis.European biophysics journal : EBJ · 2011Article
- Biophysical characterization of a recombinant α-amylase from thermophilic Bacillus sp. strain TS-23.The protein journal · 2010Article
- Metal-controlled interdomain cooperativity in parvalbumins.Cell calcium · 2009Article
- Carboxylic ester hydrolases from hyperthermophiles.Extremophiles : life under extreme conditions · 2009Review
- Structural determinants of the high thermal stability of SsoPox from the hyperthermophilic archaeon Sulfolobus solfataricus.Extremophiles : life under extreme conditions · 2009Article
- Rett syndrome-causing mutations in human MeCP2 result in diverse structural changes that impact folding and DNA interactions.The Journal of biological chemistry · 2008Article
- Structure and increased thermostability of Rhodococcus sp. naphthalene 1,2-dioxygenase.Journal of bacteriology · 2005Article
- Kinetic inactivation study of mushroom tyrosinase: intermediate detection by denaturants.Journal of protein chemistry · 2003Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The stability of two thermophilic esterases, AFEST from Archaeoglobus fulgidus and EST2 from Alicyclobacillus acidocaldarius, against the denaturing action of urea and guanidine hydrochloride has been investigated by means of steady-state fluorescence and circular dichroism measurements. Experimental results indicate that the two enzymes, even though very resistant to temperature and urea, show a resistance to guanidine hydrochloride weaker than expected on the basis of data collected so far for a large set of globular proteins. Structural information available for AFEST and EST2 and ideas that emerged from studies on the molecular origin of the greater thermal stability of thermophiles allow the suggestion of a reliable rationale. The present results may be an indication that the optimization of charge-charge interactions on the protein surface is a key factor for the stability of the two esterases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.