ArticleThe Journal of physiology2002
Passive water and urea permeability of a human Na(+)-glutamate cotransporter expressed in Xenopus oocytes.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Honey, I shrunk the extracellular space: Measurements and mechanisms of astrocyte swelling.Glia · 2022Review
- Review
- Ataxia-linked SLC1A3 mutations alter EAAT1 chloride channel activity and glial regulation of CNS function.The Journal of clinical investigation · 2022Article
- Molecular mechanisms of brain water transport.Nature reviews. Neuroscience · 2021Review
- Compromised Astrocyte Swelling/Volume Regulation in the Hippocampus of the Triple Transgenic Mouse Model of Alzheimer's Disease.Frontiers in aging neuroscience · 2021Article
- Role of Glutamatergic Excitotoxicity in Neuromyelitis Optica Spectrum Disorders.Frontiers in cellular neuroscience · 2019Review
- Article
- Physiology of Astroglia.Physiological reviews · 2018Review
- Structural and functional significance of water permeation through cotransporters.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Potential Roles of Aquaporin 4 in Alzheimer's Disease.Molecular neurobiology · 2016Review
- Formation of a Chloride-conducting State in the Maltose ATP-binding Cassette (ABC) Transporter.The Journal of biological chemistry · 2016Article
- Molecular pathophysiology of cerebral edema.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2016Review
- The Split Personality of Glutamate Transporters: A Chloride Channel and a Transporter.Neurochemical research · 2016Article
- The Neuroprotective Effect of the Association of Aquaporin-4/Glutamate Transporter-1 against Alzheimer's Disease.Neural plasticity · 2016Review
- Transient formation of water-conducting states in membrane transporters.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Glial K⁺ clearance and cell swelling: key roles for cotransporters and pumps.Neurochemical research · 2012Review
- Water permeation through the sodium-dependent galactose cotransporter vSGLT.Biophysical journal · 2010Article
- Oligomeric structure and functional characterization of the urea transporter from Actinobacillus pleuropneumoniae.Journal of molecular biology · 2009Article
- Astrocyte and neuron intone through glutamate.Neurochemical research · 2008Review
- Water permeability of Na+-K+-2Cl- cotransporters in mammalian epithelial cells.The Journal of physiology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human Na(+)-glutamate transporter (EAAT1) was expressed in Xenopus laevis oocytes. The passive water permeability, L(p), was derived from volume changes of the oocyte induced by changes in the external osmolarity. Oocytes were subjected to two-electrode voltage clamp. In the presence of Na(+), the EAAT1-specific (defined in Discussion) L(p) increased linearly with positive clamp potentials, the L(p) being around 23 % larger at +50 mV than at -50 mV. L-Glutamate increased the EAAT1-specific L(p) by up to 40 %. The K(0.5) for the glutamate-dependent increase was 20 +/- 6 microM, which is similar to the K(0.5) value for glutamate activation of transport. The specific inhibitor DL-threo-beta-benzyloxyaspartate (TBOA) reduced the EAAT1-specific L(p) to 72 %. EAAT1 supported passive fluxes of [(14)C]urea and [(14)C]glycerol. The [(14)C]urea flux was increased in the presence of glutamate. The data suggest that the permeability depends on the conformational equilibrium of the EAAT1. At positive potentials and in the presence of Na(+) and glutamate, the pore is enlarged and water and urea penetrate more readily. The L(p) was larger when measured with urea or glycerol as osmolytes as compared with mannitol. Apparently, the properties of the pore are not uniform along its length. The outer section may accommodate urea and glycerol in an osmotically active form, giving rise to larger water fluxes. The physiological role of EAAT1 for water homeostasis in the central nervous system is discussed.
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