ArticleEnvironmental health perspectives2002
Dual effects of phytoestrogens result in u-shaped dose-response curves.
Article in Environmental health perspectives, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 162 citations in OpenAlex.
- Disruptive effects of genistein and daidzein on steroidogenesis, testicular development and maturation of Cyprinus carpio (Linn, 1758) males.Fish physiology and biochemistry · 2025Article
- Role of Seed Therapy on Estrous and Non-Estrous Cycle in Healthy Female Rats.Food science & nutrition · 2025Article
- Hemostatic Effects of Raloxifene in Ovariectomized Rats.Life (Basel, Switzerland) · 2023Article
- Analytics, Properties and Applications of Biologically Active Stilbene Derivatives.Molecules (Basel, Switzerland) · 2023Review
- Review
- Bitter Taste Receptors and Endocrine Disruptors: Cellular and Molecular Insights from an In Vitro Model of Human Granulosa Cells.International journal of molecular sciences · 2022Article
- Manipulation of Dietary Intake on Changes in Circulating Testosterone Concentrations.Nutrients · 2021Review
- Epigenetic immunomodulatory effect of eugenol and astaxanthin on doxorubicin cytotoxicity in hormonal positive breast Cancer cells.BMC pharmacology & toxicology · 2021Article
- Inhibitory effect of chrysin on estrogen biosynthesis by suppression of enzyme aromatase (CYP19): A systematic review.Heliyon · 2020Review
- Perspectives Regarding the Role of Biochanin A in Humans.Frontiers in pharmacology · 2019Review
- Highly Cancer Selective Antiproliferative Activity of Natural Prenylated Flavonoids.Molecules (Basel, Switzerland) · 2018Article
- The Role of Polybrominated Diphenyl Ethers in Thyroid Carcinogenesis: Is It a Weak Hypothesis or a Hidden Reality? From Facts to New Perspectives.International journal of environmental research and public health · 2018Review
- Human cytochrome P450 enzymes 5-51 as targets of drugs and natural and environmental compounds: mechanisms, induction, and inhibition - toxic effects and benefits.Drug metabolism reviews · 2018Review
- Design considerations and analysis planning of a phase 2a proof of concept study in rheumatoid arthritis in the presence of possible non-monotonicity.BMC medical research methodology · 2017Article
- Coumestrol inhibits autoantibody production through modulating Th1 response in experimental autoimmune thyroiditis.Oncotarget · 2016Article
- The effects of the standardized extracts of Ginkgo biloba on steroidogenesis pathways and aromatase activity in H295R human adrenocortical carcinoma cells.Environmental health and toxicology · 2016Article
- Profiling of the Tox21 10K compound library for agonists and antagonists of the estrogen receptor alpha signaling pathway.Scientific reports · 2014Article
- β-D-glucan inhibits endocrine-resistant breast cancer cell proliferation and alters gene expression.International journal of oncology · 2014Article
- Colloidal drug formulations can explain "bell-shaped" concentration-response curves.ACS chemical biology · 2014Article
- Expressomal approach for comprehensive analysis and visualization of ligand sensitivities of xenoestrogen responsive genes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endocrine disruptors can affect the endocrine system without directly interacting with receptors, for example, by interfering with the synthesis or metabolism of steroid hormones. The aromatase that converts testosterone to 17beta-estradiol is a possible target. In this paper we describe an assay that simultaneously detects aromatase inhibition and estrogenicity. The principle is similar to that of other MCF-7 estrogenicity assays, but with a fixed amount of testosterone added. The endogenous aromatase activity in MCF-7 cells converts some of the testosterone to 17beta-estradiol, which is assayed by quantifying differences in the expression level of the estrogen-induced pS2 mRNA. Potential aromatase inhibitors can be identified by a dose-dependent reduction in the pS2 mRNA expression level after exposure to testosterone and the test compound. Using this assay, we have investigated several compounds, including synthetic chemicals and phytoestrogens, for aromatase inhibition. The phytoestrogens, except genistein, were aromatase inhibitors at low concentrations (< 1 micro M) but estrogenic at higher concentrations (greater than or equal to 1 micro M), resulting in U-shaped dose-response curves. None of the tested synthetic chemicals were aromatase inhibitors. The low-dose aromatase inhibition distinguished phytoestrogens from other estrogenic compounds and may partly explain reports about antiestrogenic properties of phytoestrogens. Aromatase inhibition may play an important role in the protective effects of phytoestrogens against breast cancer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.