ArticleProceedings of the National Academy of Sciences of the United States of America2002
Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 266 papers.
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Who cites it
266 citing papers in PubMed, 563 citations in OpenAlex.
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- Genome-wide DNA methylation landscape and its association with the transcriptome reprogramming in potato in response toHorticulture research · 2026Article
- Identification of DNA Methyltransferase/Demethylase Genes and 5-Azacytidine's Impact on β-Elemene and Methylation inBiology · 2025Article
- Major alleles of CDCA7 shape CG methylation in Arabidopsis thaliana.Nature plants · 2025Article
- Homeostasis of DNA Hemi-Methylation in Arabidopsis through Methylation Maintenance, DNA Replication, and Nucleosome Positioning Mechanisms.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Delay in Flowering Time inInternational journal of molecular sciences · 2025Article
- Genome-Wide Identification of DNA Methyltransferase and Demethylase inPlants (Basel, Switzerland) · 2025Article
- Epigenetic insights into the domestication of tetraploid peanut.Plant physiology · 2025Article
- Molecular Mechanisms Underlying the Establishment, Maintenance, and Removal of DNA Methylation in Plants.Annual review of plant biology · 2025Review
- Alternative silencing states of transposable elements in Arabidopsis associated with H3K27me3.Genome biology · 2025Article
- Epigenetic odyssey to decrypt the hidden code for sustainable brassica production: enhancing yield, stress resilience and nutritional quality.Frontiers in plant science · 2025Review
- Population-wide DNA methylation polymorphisms at single-nucleotide resolution in 207 cotton accessions reveal epigenomic contributions to complex traits.Cell research · 2024Article
- Genome-Wide Screening and Characterization of Methyl-CpG-Binding Domain (MBD) Proteins inCurrent issues in molecular biology · 2024Article
- Unveiling the role of epigenetics in leaf senescence: a comparative study to identify different epigenetic regulations of senescence types in barley leaves.BMC plant biology · 2024Article
- Non-CG DNA hypomethylation promotes photosynthesis and nitrogen fixation in soybean.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Non-canonical bases differentially represented in the sex chromosomes of the dioecious plant Silene latifolia.Journal of experimental botany · 2024Article
- A DNA demethylase reduces seed size by decreasing the DNA methylation of AT-rich transposable elements in soybean.Communications biology · 2024Article
- Molecular Evolution of RAMOSA1 (RA1) in Land Plants.Biomolecules · 2024Article
- Reciprocal expression of MADS-box genes and DNA methylation reconfiguration initiate bisexual cones in spruce.Communications biology · 2024Article
206 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many plant, animal, and fungal genomes contain cytosine DNA methylation in asymmetric sequence contexts (CpHpH, H = A, T, C). Although the enzymes responsible for this methylation are unknown, it has been assumed that asymmetric methylation is maintained by the persistent activity of de novo methyltransferases (enzymes capable of methylating previously unmodified DNA). We recently reported that the DOMAINS REARRANGED METHYLASE (DRM) genes are required for de novo DNA methylation in Arabidopsis thaliana because drm1 drm2 double mutants lack the de novo methylation normally associated with transgene silencing. In this study, we have used bisulfite sequencing and Southern blot analysis to examine the role of the DRM loci in the maintenance of asymmetric methylation. At some loci, drm1 drm2 double mutants eliminated all asymmetric methylation. However, at the SUPERMAN locus, asymmetric methylation was only completely abolished in drm1 drm2 chromomethylase 3 (cmt3) triple mutant plants. drm1 drm2 double mutants also showed a strong reduction of CpNpG (n = A, T, C, or G) methylation at some loci, but not at others. The drm1 drm2 cmt3 triple mutant plants did not affect CpG methylation at any locus tested, suggesting that the primary CpG methylases are encoded by the MET1 class of genes. Although neither the drm1 drm2 double mutants nor the cmt3 single mutants show morphological defects, drm1 drm2 cmt3 triple mutant plants show pleiotropic effects on plant development. Our results suggest that the DRM and CMT3 genes act in a partially redundant and locus-specific manner to control asymmetric and CpNpG methylation.
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