ArticleProceedings of the National Academy of Sciences of the United States of America2002
Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 82 citations in OpenAlex.
- 4D single-cell spatial transcriptomics reveals dynamic morphogenetic gradients and regenerative domains in planarians.GigaScience · 2026Article
- Targeting MED8 enhances sorafenib sensitivity in hepatocellular carcinoma by disrupting epithelial-mesenchymal transition mechanisms.Journal of enzyme inhibition and medicinal chemistry · 2025Article
- Regulatory Mechanisms of miRNA Turnover: Insights into ZSWIM8-Mediated Target-Directed MicroRNA Degradation.Biomedicines · 2025Review
- The functions and properties of cullin-5, a potential therapeutic target for cancers.American journal of translational research · 2020Review
- Proteins interacting with CreA and CreB in the carbon catabolite repression network in Aspergillus nidulans.Current genetics · 2017Article
- EPOP Interacts with Elongin BC and USP7 to Modulate the Chromatin Landscape.Molecular cell · 2016Article
- Rift Valley fever virus NSs protein functions and the similarity to other bunyavirus NSs proteins.Virology journal · 2016Review
- Disordered proteinaceous machines.Chemical reviews · 2014Review
- The elongin complex antagonizes the chromatin factor Corto for vein versus intervein cell identity in Drosophila wings.PloS one · 2013Article
- Article
- Proteasome-mediated turnover of Arabidopsis MED25 is coupled to the activation of FLOWERING LOCUS T transcription.Plant physiology · 2012Article
- Ubiquitin and proteasomes in transcription.Annual review of biochemistry · 2012Review
- Elongin C is a mediator of Notch4 activity in human renal tubule cells.Biochimica et biophysica acta · 2011Article
- Knockdown of NtMed8, a Med8-like gene, causes abnormal development of vegetative and floral organs in tobacco (Nicotiana tabacum L.).Plant cell reports · 2011Article
- Mediator and human disease.Seminars in cell & developmental biology · 2011Review
- Origins and activity of the Mediator complex.Seminars in cell & developmental biology · 2011Review
- Screening and identification of host factors interacting with UL14 of herpes simplex virus 1.Medical microbiology and immunology · 2011Article
- NSs protein of rift valley fever virus promotes posttranslational downregulation of the TFIIH subunit p62.Journal of virology · 2011Article
- Szt2, a novel gene for seizure threshold in mice.Genes, brain, and behavior · 2009Article
- The F-box protein beta-TrCp1/Fbw1a interacts with p300 to enhance beta-catenin transcriptional activity.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
Abstract
The heterodimeric Elongin BC complex has been shown to interact in vitro and in cells with a conserved BC-box motif found in an increasing number of proteins including RNA polymerase II elongation factor Elongin A, suppressor of cytokine signaling (SOCS)-box proteins, and the von Hippel-Lindau tumor suppressor protein. Recently, the Elongin BC complex was found to function as an adaptor that links these BC-box proteins to a module composed of Cullin family members Cul2 or Cul5 and RING-H2 finger protein Rbx1 to reconstitute a family of E3 ubiquitin ligases that activate ubiquitylation by the E2 ubiquitin-conjugating enzyme Ubc5. As part of our effort to understand the functions of Elongin BC-based ubiquitin ligases, we exploited a modified yeast two-hybrid screen to identify a mammalian BC-box protein similar in sequence to Saccharomyces cerevisiae Mediator subunit Med8p. In this report we demonstrate (i) that mammalian MED8 is a subunit of the mammalian Mediator complex and (ii) that MED8 can assemble with Elongins B and C, Cul2, and Rbx1 to reconstitute a ubiquitin ligase. Taken together, our findings are consistent with the model that MED8 could function to recruit ubiquitin ligase activity directly to the RNA polymerase II transcriptional machinery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.