ArticleProceedings of the National Academy of Sciences of the United States of America2002
Absolute requirement of spermidine for growth and cell cycle progression of fission yeast (Schizosaccharomyces pombe).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Evolution of polyamine resistance inmSphere · 2025Article
- Defence Warriors: Exploring the crosstalk between polyamines and oxidative stress during microbial pathogenesis.Redox biology · 2025Review
- Evolution of polyamine resistance inbioRxiv : the preprint server for biology · 2024Article
- Exploring polyamine metabolism of the yeast-like fungus, Emergomyces africanus.FEMS yeast research · 2024Article
- Optimized sample buffer for dispersed, high-resolution capillary zone electrophoretic separation of Escherichia coli B.Scientific reports · 2023Article
- New Insights into the Roles and Mechanisms of Spermidine in Aging and Age-Related Diseases.Aging and disease · 2021Review
- Interactions between the 5' UTR mRNA of theRNA (New York, N.Y.) · 2020Article
- Hypusine, a polyamine-derived amino acid critical for eukaryotic translation.The Journal of biological chemistry · 2018Review
- Coupling of the polyamine and iron metabolism pathways in the regulation of proliferation: Mechanistic links to alterations in key polyamine biosynthetic and catabolic enzymes.Biochimica et biophysica acta. Molecular basis of disease · 2018Article
- Spermine modulates fungal morphogenesis and activates plasma membrane HBiology open · 2018Article
- The roles of polyamines in microorganisms.World journal of microbiology & biotechnology · 2017Review
- Fission Yeast srm1 is Involved in Stress Response and Cell Cycle.Current microbiology · 2017Article
- Gamma-Glutamylpolyamine Synthetase GlnA3 Is Involved in the First Step of Polyamine Degradation Pathway inFrontiers in microbiology · 2017Article
- The Aspergillus fumigatus cell wall integrity signaling pathway: drug target, compensatory pathways, and virulence.Frontiers in microbiology · 2015Review
- A genome-wide screen for sporulation-defective mutants in Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2014Article
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- Legionella pneumophila requires polyamines for optimal intracellular growth.Journal of bacteriology · 2011Article
- Microarray studies on the genes responsive to the addition of spermidine or spermine to a Saccharomyces cerevisiae spermidine synthase mutant.Yeast (Chichester, England) · 2009Article
- Plant polyamine catabolism: The state of the art.Plant signaling & behavior · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Schizosaccharomyces pombe cells that cannot synthesize spermidine or spermine because of a deletion-insertion in the gene coding for S-adenosylmethionine decarboxylase (Deltaspe2) have an absolute requirement for spermidine for growth. Flow cytometry studies show that in the absence of spermidine an overall delay of the cell cycle progression occurs with some accumulation of cells in the G(1) phase; as little as 10(-6) M spermidine is sufficient to maintain normal cell cycle distribution and normal growth. Morphologically some of the spermidine-deprived cells become spherical at an early stage with little evidence of cell division. On further incubation in the spermidine-deprived medium, growth occurs in most of the cells, not by cell division but rather by cell elongation, with an abnormal distribution of the actin cytoskeleton, DNA (4', 6-diamidino-2-phenylindole staining), and calcofluor-staining moieties. More prolonged incubation in the spermidine-deficient medium leads to profound morphological changes including nuclear degeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.