ArticleProceedings of the National Academy of Sciences of the United States of America2002
NotI subtraction and NotI-specific microarrays to detect copy number and methylation changes in whole genomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Integrin α9 gene promoter is hypermethylated and downregulated in nasopharyngeal carcinoma.Oncotarget · 2015Article
- Identification of Novel Epigenetic Markers of Prostate Cancer by NotI-Microarray Analysis.Disease markers · 2015Article
- Epigenetic alterations of chromosome 3 revealed by NotI-microarrays in clear cell renal cell carcinoma.BioMed research international · 2014Article
- Novel tumor suppressor candidates on chromosome 3 revealed by NotI-microarrays in cervical cancer.Epigenetics · 2013Article
- Article
- NotI microarrays: novel epigenetic markers for early detection and prognosis of high grade serous ovarian cancer.International journal of molecular sciences · 2012Article
- Anti-angiogenic and tumor-suppressive roles of candidate tumor-suppressor gene, Fibulin-2, in nasopharyngeal carcinoma.Oncogene · 2012Article
- Article
- Simultaneous down-regulation of tumor suppressor genes RBSP3/CTDSPL, NPRL2/G21 and RASSF1A in primary non-small cell lung cancer.BMC cancer · 2010Article
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- Article
- Microarray-based DNA methylation profiling: technology and applications.Nucleic acids research · 2006Article
- Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR.Plant cell reports · 2005Article
- RBSP3 (HYA22) is a tumor suppressor gene implicated in major epithelial malignancies.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Restriction site tagged (RST) microarrays: a novel technique to study the species composition of complex microbial systems.Nucleic acids research · 2003Article
- NotI passporting to identify species composition of complex microbial systems.Nucleic acids research · 2003Article
- Tumor suppressor genes on chromosome 3p involved in the pathogenesis of lung and other cancers.Oncogene · 2002Review
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Authors and funding
17 authors.
Funding
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Abstract
Methylation, deletions, and amplifications of cancer genes constitute important mechanisms in carcinogenesis. For genome-wide analysis of these changes, we propose the use of NotI clone microarrays and genomic subtraction, because NotI recognition sites are closely associated with CpG islands and genes. We show here that the CODE (Cloning Of DEleted sequences) genomic subtraction procedure can be adapted to NotI flanking sequences and to CpG islands. Because the sequence complexity of this procedure is greatly reduced, only two cycles of subtraction are required. A NotI-CODE procedure can be used to prepare NotI representations (NRs) containing 0.1-0.5% of the total DNA. The NRs contain, on average, 10-fold less repetitive sequences than the whole human genome and can be used as probes for hybridization to NotI microarrays. These microarrays, when probed with NRs, can simultaneously detect copy number changes and methylation. NotI microarrays offer a powerful tool with which to study carcinogenesis.
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