ArticleJournal of clinical microbiology2002
Development of a rapid real-time PCR assay for quantitation of Pneumocystis carinii f. sp. carinii.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00342589 (Studies of Human Pneumocystis Infection), which is not on this map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Studies of Human Pneumocystis Infection
Who cites it
19 citing papers in PubMed.
- Diagnosis ofInfection & chemotherapy · 2025Review
- Metabolic modelling as a powerful tool to identify critical components of Pneumocystis growth medium.PLoS computational biology · 2024Article
- Axenic Long-Term Cultivation ofJournal of fungi (Basel, Switzerland) · 2023Article
- The Persistent Challenge of Pneumocystis Growth Outside the Mammalian Lung: Past and Future Approaches.Frontiers in microbiology · 2021Review
- Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2018Article
- Usefulness of 1,3 Beta-D-Glucan Detection in non-HIV Immunocompromised Mechanical Ventilated Critically Ill Patients with ARDS and Suspected Pneumocystis jirovecii Pneumonia.Mycopathologia · 2017Article
- Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study.EBioMedicine · 2017Observational
- Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts.Nature communications · 2016Article
- Quantitative real-time PCR (qPCR) for Eimeria tenella replication--Implications for experimental refinement and animal welfare.Parasitology international · 2015Article
- Sequencing and characterization of the complete mitochondrial genomes of three Pneumocystis species provide new insights into divergence between human and rodent Pneumocystis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Validation of the MycAssay Pneumocystis kit for detection of Pneumocystis jirovecii in bronchoalveolar lavage specimens by comparison to a laboratory standard of direct immunofluorescence microscopy, real-time PCR, or conventional PCR.Journal of clinical microbiology · 2012Article
- Clinical significance of quantifying Pneumocystis jirovecii DNA by using real-time PCR in bronchoalveolar lavage fluid from immunocompromised patients.Journal of clinical microbiology · 2012Article
- Complexity of the MSG gene family of Pneumocystis carinii.BMC genomics · 2009Article
- Detection of Pneumocystis jirovecii by two staining methods and two quantitative PCR assays.Infection · 2009Article
- Pneumocystis murina MSG gene family and the structure of the locus associated with its transcription.Fungal genetics and biology : FG & B · 2007Article
- Primary pneumocystis infection in infants hospitalized with acute respiratory tract infection.Emerging infectious diseases · 2007Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Noninvasive method for monitoring Pneumocystis carinii pneumonia.Emerging infectious diseases · 2003Article
- Comparison of quantitative reverse transcription-PCR to viral culture for assessment of respiratory syncytial virus shedding.Journal of clinical microbiology · 2003Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
A method for reliable quantification of Pneumocystis carinii in research models of P. carinii pneumonia (PCP) that is more convenient and reproducible than microscopic enumeration of organisms would greatly facilitate investigations of this organism. We developed a rapid quantitative touchdown (QTD) PCR assay for detecting P. carinii f. sp. carinii, the subspecies of P. carinii commonly used in research models of PCP. The assay was based on the single-copy dihydrofolate reductase gene and was able to detect <5 copies of a plasmid standard per tube. It was reproducibly quantitative (r = 0.99) over 6 log values for standards containing > or =5 copies/tube. Application of the assay to a series of 10-fold dilutions of P. carinii organisms isolated from rat lung demonstrated that it was reproducibly quantitative over 5 log values (r = 0.99). The assay was applied to a recently reported in vitro axenic cultivation system for P. carinii and confirmed our microscopy findings that no organism multiplication had occurred during culture. For all cultures analyzed, QTD PCR assays showed a decrease in P. carinii DNA that exceeded the expected decrease due to dilution of the inoculum upon transfer. In conclusion, a rapid, sensitive, and reproducible quantitative PCR assay for P. carinii f. sp. carinii has been developed and is applicable to in vivo as well as in vitro systems. The assay should prove useful for conducting studies in which quantification of organism burden or growth assessment is critical, such as in vitro antimicrobic susceptibility testing or in vivo immunopathological experiments.
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