ArticleJournal of clinical microbiology2002
Genotyping of Francisella tularensis strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- 16S rDNA and ITS Sequence Diversity of Burkholderia mallei Isolated from Glanders-Affected Horses and Mules in India (2013-2019).Current microbiology · 2021Article
- Tularemia Outbreaks in Spain from 2007 to 2020 in Humans and Domestic and Wild Animals.Pathogens (Basel, Switzerland) · 2021Article
- Phylogenetic Lineages ofFrontiers in cellular and infection microbiology · 2018Review
- Rapid Identification and Characterization of Francisella by Molecular Biology and Other Techniques.The open microbiology journal · 2016Article
- Molecular investigation of tularemia outbreaks, Spain, 1997-2008.Emerging infectious diseases · 2014Article
- Insights to genetic characterization tools for epidemiological tracking of Francisella tularensis in Sweden.PloS one · 2014Article
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- Investigation of tularemia outbreak after natural infection of outdoor-housed rhesus macaques (Macaca mulatta) with Francisella tularensis.Comparative medicine · 2013Article
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- Phylogeography of Francisella tularensis: global expansion of a highly fit clone.Journal of bacteriology · 2009Article
- Rapid comparative genomic analysis for clinical microbiology: the Francisella tularensis paradigm.Genome research · 2008Article
- Canonical insertion-deletion markers for rapid DNA typing of Francisella tularensis.Emerging infectious diseases · 2007Article
- Genomic deletion marking an emerging subclone of Francisella tularensis subsp. holarctica in France and the Iberian Peninsula.Applied and environmental microbiology · 2007Article
- Comparison of Francisella tularensis genomes reveals evolutionary events associated with the emergence of human pathogenic strains.Genome biology · 2007Article
- Paired-end sequence mapping detects extensive genomic rearrangement and translocation during divergence of Francisella tularensis subsp. tularensis and Francisella tularensis subsp. holarctica populations.Journal of bacteriology · 2006Article
- Epidemiologic and molecular analysis of human tularemia, United States, 1964-2004.Emerging infectious diseases · 2006Article
- Worldwide genetic relationships among Francisella tularensis isolates determined by multiple-locus variable-number tandem repeat analysis.Journal of bacteriology · 2004Article
- Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F. tularensis subsp. tularensis.Journal of clinical microbiology · 2003Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluated three molecular methods for identification of Francisella strains: pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) analysis, and 16S rRNA gene sequencing. The analysis was performed with 54 Francisella tularensis subsp. holarctica, 5 F. tularensis subsp. tularensis, 2 F. tularensis subsp. novicida, and 1 F. philomiragia strains. On the basis of the combination of results obtained by PFGE with the restriction enzymes XhoI and BamHI, PFGE revealed seven pulsotypes, which allowed us to discriminate the strains to the subspecies level and which even allowed us to discriminate among some isolates of F. tularensis subsp. holarctica. The AFLP analysis technique produced some degree of discrimination among F. tularensis subsp. holarctica strains (one primary cluster with three major subclusters and minor variations within subclusters) when EcoRI-C and MseI-A, EcoRI-T and MseI-T, EcoRI-A and MseI-C, and EcoRI-0 and MseI-CA were used as primers. The degree of similarity among the strains was about 94%. The percent similarities of the AFLP profiles of this subspecies compared to those of F. tularensis subsp. tularensis, F. tularensis subsp. novicida, and F. philomiragia were less than 90%, about 72%, and less than 24%, respectively, thus permitting easy differentiation of this subspecies. 16S rRNA gene sequencing revealed 100% similarity for all F. tularensis subsp. holarctica isolates compared in this study. These results suggest that although limited genetic heterogeneity among F. tularensis subsp. holarctica isolates was observed, PFGE and AFLP analysis appear to be promising tools for the diagnosis of infections caused by different subspecies of F. tularensis and suitable techniques for the differentiation of individual strains.
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