ArticleJournal of clinical microbiology2002
Multiplex PCR assays for simultaneous detection of six major serotypes and two virulence-associated phenotypes of Streptococcus suis in tonsillar specimens from pigs.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 75 citations in OpenAlex.
- The kinetics of maternal and self-developed Streptococcus suis-specific antibodies.Porcine health management · 2025Article
- Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection.Veterinary research · 2025Article
- Genomic epidemiology inHeliyon · 2024Review
- Invasive Bacterial Infections of the Musculoskeletal and Central Nervous System during Pig Rearing: Detection Frequencies of Different Pathogens and SpecificVeterinary sciences · 2024Article
- Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2Microbial genomics · 2022Article
- A critical review speculating on the protective efficacies of autogenousPorcine health management · 2020Review
- Exploring target-specific primer extension in combination with a bead-based suspension array for multiplexed detection and typing using Streptococcus suis as a model pathogen.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
- Effect of Simultaneous Exposure of Pigs to Streptococcus suis Serotypes 2 and 9 on Their Colonization and Transmission, and on Mortality.Pathogens (Basel, Switzerland) · 2017Article
- Serotypes of Streptococcus suis isolated from healthy pigs in Phayao Province, Thailand.BMC research notes · 2017Article
- An emerging zoonotic clone in the Netherlands provides clues to virulence and zoonotic potential of Streptococcus suis.Scientific reports · 2016Article
- Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-Time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs.Pathogens (Basel, Switzerland) · 2016Article
- Current Taxonomical Situation of Streptococcus suis.Pathogens (Basel, Switzerland) · 2016Review
- Identification and Characterization of IgdE, a Novel IgG-degrading Protease of Streptococcus suis with Unique Specificity for Porcine IgG.The Journal of biological chemistry · 2016Article
- Genetic diversity of Streptococcus suis serotype 2 isolated from pigs in Brazil.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Article
- Genetic diversity and virulence of novel sequence types of Streptococcus suis from diseased and healthy pigs in China.Frontiers in microbiology · 2015Article
- Assessment of MALDI-TOF MS as Alternative Tool for Streptococcus suis Identification.Frontiers in public health · 2015Article
- Increased production of suilysin contributes to invasive infection of the Streptococcus suis strain 05ZYH33.Molecular medicine reports · 2014Article
- Streptococcus suis, an important pig pathogen and emerging zoonotic agent-an update on the worldwide distribution based on serotyping and sequence typing.Emerging microbes & infections · 2014Review
- Streptococcus suis infection: an emerging/reemerging challenge of bacterial infectious diseases?Virulence · 2014Review
- Characterization of Streptococcus suis isolates from slaughter swine.Current microbiology · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiplex PCR assays for the detection and identification of various Streptococcus suis strains in tonsillar specimens from pigs were developed and evaluated. In two separate reactions, five distinct DNA targets were amplified. Three targets, based on the S. suis capsular polysaccharide (cps) genes specific for serotypes 1 (and 14), 7, and 9, were amplified in multiplex PCR I. Two other targets, based on the serotype 2- (and 1/2-) specific cps gene and the epf gene, encoding the EF proteins of virulent serotype 2 and highly virulent serotype 1 strains, were amplified in multiplex PCR II. To identify false-negative results, firefly luciferase (luc) DNA and primers based on the luc gene were included in the assay. The multiplex PCR assays were evaluated with tonsillar specimens from pigs infected with S. suis strains. The results obtained with the PCR assays were compared with the results obtained with a bacteriological examination. Most (94%) of the results obtained with multiplex PCR assays were confirmed by the bacteriological examination. The PCR method seems to be more sensitive compared to the bacteriological method, since the remaining 6% of the samples were positive by PCR and negative by bacteriological examination. These results indicate that the PCR method is highly specific for the detection of S. suis strains most frequently involved in clinical disease in infected pig herds. The serotypes found by PCR in tonsillar specimens from diseased pigs were compared with the serotypes of the strains isolated from the affected tissues of the same pigs. The results showed that there is significant association between carriership and clinical illness for S. suis serotype 9 and EF-positive serotype 2 strains and not for serotype 7 and EF-negative serotype 2 (or 1/2) strains.
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