ArticleJournal of clinical microbiology2002
Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
81 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Molecular quantification and differentiation of Candida species in biological specimens of patients with liver cirrhosis.PloS one · 2018Trial
- Using Phenotypic, Biochemical, and Molecular Methods to IdentifyEpidemiologia (Basel, Switzerland) · 2026Article
- Development and validation of conventional and TaqMan real-time PCR for the detection of Trichoderma afroharzianum causing corn ear rot.Scientific reports · 2026Article
- Antifungal activity of curcumin nanoparticles and Saccharomyces cerevisiae against aflatoxigenic Aspergillus flavus isolated from broiler chickens rations in Egypt.BMC microbiology · 2026Article
- Article
- Design of a melting curve analysis (MCA) based on multiplex real-time PCR for detection of Aspergillus terreus and Aspergillus fumigatus in cereals and oilseeds samples.BMC research notes · 2025Article
- Characterization and virulence of yeasts associated with neonatal thrush.BMC microbiology · 2025Article
- Mitigating drought stress and enhancing maize resistance through biopriming with Rhizopus arrhizus: insights into Morpho-Biochemical and molecular adjustments.BMC plant biology · 2025Article
- A novel clotrimazole selenium nano-composite for combating deep dermal Candida albicans infections and virulence genes.The Journal of antibiotics · 2025Article
- Vaginal Microbiota Patterns Associated with Yeast Infection in Mexican Women, a Pilot Study.Biotech (Basel (Switzerland)) · 2025Article
- Identification and antifungal susceptibility patterns of reference yeast strains to novel and conventional agents: a comparative study using CLSI, EUCAST and Sensititre YeastOne methods.JAC-antimicrobial resistance · 2025Article
- Molecular characterization of some multidrug resistant Candida Auris in egypt.Scientific reports · 2025Article
- Article
- Review
- A Comprehensive Review of Identification Methods for Pathogenic Yeasts: Challenges and Approaches.Advanced biomedical research · 2023Review
- High Virulence and Multidrug Resistance ofMicroorganisms · 2022Article
- Multiplex PCR Identification ofFoods (Basel, Switzerland) · 2022Article
- Anti-biofilm properties of eucalyptol in combination with antifungals against Candida albicans isolates in patients with hematological malignancy.Archives of microbiology · 2022Article
- Application of Two-Photon Microscopy to Study Sclerotium cepivorum Berk Sclerotia Isolated from Naturally Infested Soil and Produced In Vitro.Current microbiology · 2021Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
A multiplex PCR method was developed to identify simultaneously multiple fungal pathogens in a single reaction. Five sets of species-specific primers were designed from the internal transcribed spacer (ITS) regions, ITS1 and ITS2, of the rRNA gene to identify Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, and Aspergillus fumigatus. Another set of previously published ITS primers, CN4 and CN5, were used to identify Cryptococcus neoformans. Three sets of primers were used in one multiplex PCR to identify three different species. Six different species of pathogenic fungi can be identified with two multiplex PCRs. Furthermore, instead of using templates of purified genomic DNA, we performed the PCR directly from yeast colonies or cultures, which simplified the procedure and precluded contamination during the extraction of DNA. A total of 242 fungal isolates were tested, representing 13 species of yeasts, four species of Aspergillus, and three zygomycetes. The multiplex PCR was tested on isolated DNA or fungal colonies, and both provided 100% sensitivity and specificity. However, DNA from only about half the molds could be amplified directly from mycelial fragments, while DNA from every yeast colony was amplified. This multiplex PCR method provides a rapid, simple, and reliable alternative to conventional methods to identify common clinical fungal isolates.
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