ArticleJournal of clinical microbiology2002
Alveolar echinococcosis: characterization of diagnostic antigen Em18 and serological evaluation of recombinant Em18.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 81 citations in OpenAlex.
- Correlation between imaging features and rEm18 antibodies in alveolar echinococcosis: results from a multicenter study in France.Parasite (Paris, France) · 2025Trial
- The establishment of a universal blocking ELISA for echinococcosis using immunoglobulin against a new conserved linear B-cell epitope.Microbiology spectrum · 2026Article
- Inflammatory exacerbation of hepatic alveolar echinococcosis during dupilumab therapy: A case report.IDCases · 2026Article
- Advances in Novel Diagnostic Techniques for Alveolar Echinococcosis.Diagnostics (Basel, Switzerland) · 2025Review
- Monoclonal antibody-based localization of major diagnostic antigens in metacestode tissue, excretory/secretory products, and extracellular vesicles ofFrontiers in cellular and infection microbiology · 2023Article
- Serological Assays for Alveolar and Cystic Echinococcosis-A Comparative Multi-Test Study in Switzerland and Kyrgyzstan.Pathogens (Basel, Switzerland) · 2022Article
- Echinococcus granulosus Protoscolex DM9 Protein Shows High Potential for Serodiagnosis of Alveolar Echinococcosis.The Korean journal of parasitology · 2022Article
- Evaluation of the diagnostic value of the immunoblotting and ELISA tests using recombinant Em18 antigen in human alveolar echinococcosis from Xingjiang China.Experimental and therapeutic medicine · 2018Article
- Intense Focus of Alveolar Echinococcosis, South Kyrgyzstan.Emerging infectious diseases · 2018Article
- Serological validation of an alveolar echinococcosis rat model with a single hepatic lesion.The Journal of veterinary medical science · 2017Article
- Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.PLoS neglected tropical diseases · 2016Article
- An Echinococcus multilocularis Antigen B3 Proteoform That Shows Specific Antibody Responses to Active-Stage Alveolar Echinococcosis.Journal of clinical microbiology · 2015Article
- Cystic echinococcoses in Mongolia: molecular identification, serology and risk factors.PLoS neglected tropical diseases · 2014Article
- Biochemical and molecular characterization of the tegument protein RT10 from Raillietina tetragona.Parasitology research · 2014Article
- Comparison of the serological tests ICT and ELISA for the diagnosis of alveolar echinococcosis in France.Parasite (Paris, France) · 2014Article
- Differential diagnosis of cystic and alveolar echinococcosis using an immunochromatographic test based on the detection of specific antibodies.Parasitology research · 2013Article
- Subcutaneous infection model facilitates treatment assessment of secondary Alveolar echinococcosis in mice.PLoS neglected tropical diseases · 2013Article
- Immunochromatographic test with recombinant Em18 antigen for the follow-up study of alveolar echinococcosis.Clinical and vaccine immunology : CVI · 2011Article
- Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray.PLoS neglected tropical diseases · 2010Article
- Immunoglobulin G subclass responses to recombinant Em18 in the follow-up of patients with alveolar echinococcosis in different clinical stages.Clinical and vaccine immunology : CVI · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
The Echinococcus multilocularis protein Em18 is one of the most promising antigens for use in serodiagnosis of alveolar echinococcosis in human patients. Here we identify an antigenic relationship between Em18 and a 65-kDa immunodominant E. multilocularis surface protein previously identified as either EM10 or EmII/3. The NH(2)-terminal sequence of native Em18 was determined, revealing it to be a fragment of EM10. Experiments were undertaken to investigate the effect of proteinase inhibitors on the degradation of EM10 in crude extracts of E. multilocularis protoscoleces. Em18 was found to be the product of degradation of EM10 by cysteine proteinase. A recombinant Em18 (RecEm18, derived from (349)K to (508)K of EM10) was successfully expressed by using Escherichia coli expression system and then evaluated for use in serodiagnosis of alveolar echinococcosis. RecEm18 was recognized by 27 (87.1%) and 28 (90.3%) of 31 serum samples from clinically and/or pathologically confirmed alveolar echinococcosis patients by enzyme-linked immunosorbent assay and immunoblotting, respectively. Of 33 serum samples from cystic echinococcosis patients, 1 was recorded as having a weak positive reaction to RecEm18; however, none of the serum samples which were tested from neurocysticercosis patients (n = 10) or healthy people (n = 15) showed positive reactions. RecEm18 has the potential for use in the differential serodiagnosis of alveolar echinococcosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.