ArticleThe Journal of general physiology2002
Elimination of the BK(Ca) channel's high-affinity Ca(2+) sensitivity.
Article in The Journal of general physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed.
- Structural mapping of patient-associated KCNMA1 gene variants.Biophysical journal · 2024Article
- Incorporating physics to overcome data scarcity in predictive modeling of protein function: A case study of BK channels.PLoS computational biology · 2023Article
- Incorporating physics to overcome data scarcity in predictive modeling of protein function: a case study of BK channels.bioRxiv : the preprint server for biology · 2023Article
- CaInternational journal of molecular sciences · 2023Review
- Cholesterol Inhibition of Slo1 Channels Is Calcium-Dependent and Can Be Mediated by Either High-Affinity Calcium-Sensing Site in the Slo1 Cytosolic Tail.Molecular pharmacology · 2022Article
- FeACS chemical biology · 2020Article
- Coupling of CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- From Local to Global Modeling for Characterizing Calcium Dynamics and Their Effects on Electrical Activity and Exocytosis in Excitable Cells.International journal of molecular sciences · 2019Article
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- The Maxi-K (BK) Channel Antagonist Penitrem A as a Novel Breast Cancer-Targeted Therapeutic.Marine drugs · 2018Article
- Threading the biophysics of mammalian Slo1 channels onto structures of an invertebrate Slo1 channel.The Journal of general physiology · 2017Review
- Understanding the conformational motions of RCK gating rings.The Journal of general physiology · 2017Review
- Deletion of cytosolic gating ring decreases gate and voltage sensor coupling in BK channels.The Journal of general physiology · 2017Article
- Cryo-EM structure of the open high-conductance CaNature · 2017Article
- Structural basis for gating the high-conductance CaNature · 2017Article
- Interactions of divalent cations with calcium binding sites of BK channels reveal independent motions within the gating ring.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Presynaptic BK channels control transmitter release: physiological relevance and potential therapeutic implications.The Journal of physiology · 2016Review
- BK channels: multiple sensors, one activation gate.Frontiers in physiology · 2015Review
- Modeling a Ca(2+) channel/BKCa channel complex at the single-complex level.Biophysical journal · 2014Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
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Abstract
We report here a combination of site-directed mutations that eliminate the high-affinity Ca(2+) response of the large-conductance Ca(2+)-activated K(+) channel (BK(Ca)), leaving only a low-affinity response blocked by high concentrations of Mg(2+). Mutations at two sites are required, the "Ca(2+) bowl," which has been implicated previously in Ca(2+) binding, and M513, at the end of the channel's seventh hydrophobic segment. Energetic analyses of mutations at these positions, alone and in combination, argue that the BK(Ca) channel contains three types of Ca(2+) binding sites, one of low affinity that is Mg(2+) sensitive (as has been suggested previously) and two of higher affinity that have similar binding characteristics and contribute approximately equally to the power of Ca(2+) to influence channel opening. Estimates of the binding characteristics of the BK(Ca) channel's high-affinity Ca(2+)-binding sites are provided.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.