ArticleThe Biochemical journal2002
Involvement of phospholipases D1 and D2 in sphingosine 1-phosphate-induced ERK (extracellular-signal-regulated kinase) activation and interleukin-8 secretion in human bronchial epithelial cells.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
- My Journey in Academia as a Lipid Biochemist.Cell biochemistry and biophysics · 2021Review
- Phospholipase D2 restores endothelial barrier function by promoting PTPN14-mediated VE-cadherin dephosphorylation.The Journal of biological chemistry · 2020Article
- Review
- Intestinal Epithelial Cell-Specific Deletion of PLD2 Alleviates DSS-Induced Colitis by Regulating Occludin.Scientific reports · 2017Article
- Role of lipid phosphate phosphatase 3 in human aortic endothelial cell function.Cardiovascular research · 2016Article
- Using Proteomics to Understand How Leishmania Parasites Survive inside the Host and Establish Infection.International journal of molecular sciences · 2016Review
- Article
- Article
- NOD-like receptors mediated activation of eosinophils interacting with bronchial epithelial cells: a link between innate immunity and allergic asthma.Cellular & molecular immunology · 2013Article
- Lysophospholipid receptor activation of RhoA and lipid signaling pathways.Biochimica et biophysica acta · 2013Review
- Coronin 1B regulates S1P-induced human lung endothelial cell chemotaxis: role of PLD2, protein kinase C and Rac1 signal transduction.PloS one · 2013Article
- Phospholipase D signaling mediates reactive oxygen species-induced lung endothelial barrier dysfunction.Pulmonary circulation · 2013Article
- Sphingosine-1-phosphate: a potential therapeutic target for rheumatoid arthritis.Molecular biology reports · 2011Review
- Photolysis of caged sphingosine-1-phosphate induces barrier enhancement and intracellular activation of lung endothelial cell signaling pathways.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- Phospholipase D1, but not D2, regulates protein secretion via Rho/ROCK in a Ras/Raf-independent, MEK-dependent manner in rat lacrimal gland.Investigative ophthalmology & visual science · 2011Article
- House dust mite allergen Der f 2-induced phospholipase D1 activation is critical for the production of interleukin-13 through activating transcription factor-2 activation in human bronchial epithelial cells.The Journal of biological chemistry · 2009Article
- Phospholipase D-mediated activation of IQGAP1 through Rac1 regulates hyperoxia-induced p47phox translocation and reactive oxygen species generation in lung endothelial cells.The Journal of biological chemistry · 2009Article
- Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness.Nature chemical biology · 2009Article
- Expression profiles of the mouse lung identify a molecular signature of time-to-birth.American journal of respiratory cell and molecular biology · 2009Article
- Divergent roles of sphingosine kinases in kidney ischemia-reperfusion injury.Kidney international · 2009Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Sphingosine 1-phosphate (S1P), a metabolite of sphingomyelin degradation, stimulates interleukin-8 (IL-8) secretion in human bronchial epithelial (Beas-2B) cells. The molecular mechanisms regulating S1P-mediated IL-8 secretion are yet to be completely defined. Here we provide evidence that activation of phospholipases D1 and D2 (PLD1 and PLD2) by S1P regulates the phosphorylation of extracellular-signal-regulated kinase (ERK) and IL-8 secretion in Beas-2B cells. S1P, in a time- and dose-dependent manner, enhanced the threonine/tyrosine phosphorylation of ERK. The inhibition of S1P-induced ERK phosphorylation by pertussis toxin and PD 98059 indicated coupling of S1P receptors to G(i) and the ERK signalling cascade respectively. Treatment of Beas-2B cells with butan-1-ol, but not butan-3-ol, abrogated the S1P-induced phosphorylation of Raf-1 and ERK, suggesting that PLD is involved in this activation. The roles of PLD1 and PLD2 in ERK activation and IL-8 secretion activated by S1P were investigated by infecting cells with adenoviral constructs of wild-type and catalytically inactive mutants of PLD1 and PLD2. Infection of Beas-2B cells with the wild-type constructs resulted in the activation of PLD1 and PLD2 by S1P and PMA. Also, the enhanced production of [(32)P]phosphatidic acid and [(32)P]phosphatidylbutanol in the presence of butan-1-ol and the increased phosphorylation of ERK by S1P were blocked by the catalytically inactive mutants hPLD1-K898R and mPLD2-K758R. Transient transfection of Beas-2B cells with human PLD1 and mouse PLD2 cDNAs potentiated S1P-mediated IL-8 secretion compared with vector controls. In addition, PD 98059 attenuated IL-8 secretion induced by S1P in a dose-dependent fashion. These results demonstrate that both PLD1 and PLD2 participate in S1P stimulation of ERK phosphorylation and IL-8 secretion in bronchial epithelial cells.
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