ArticleApplied and environmental microbiology2002
Detection and differentiation of chlamydiae by fluorescence in situ hybridization.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 84 citations in OpenAlex.
- Stable association of a chlamydial symbiont with the freshwater predator hydra suggests broad host potential.The ISME journal · 2026Article
- Chlamydiae as symbionts of photosynthetic dinoflagellates.The ISME journal · 2024Article
- Genome Dynamics and Temperature Adaptation During Experimental Evolution of Obligate Intracellular Bacteria.Genome biology and evolution · 2023Article
- Colocalization and potential interactions ofScience advances · 2023Article
- Genotyping of Chlamydia abortus using multiple loci variable number of tandem repeats analysis technique.BMC veterinary research · 2022Article
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- Environmental marine pathogen isolation using mesocosm culture of sharpsnout seabream: striking genomic and morphological features of novel Endozoicomonas sp.Scientific reports · 2015Article
- "Candidatus Mesochlamydia elodeae" (Chlamydiae: Parachlamydiaceae), a novel chlamydia parasite of free-living amoebae.Parasitology research · 2013Article
- Metabolic features of Protochlamydia amoebophila elementary bodies--a link between activity and infectivity in Chlamydiae.PLoS pathogens · 2013Article
- Candidatus Syngnamydia venezia, a novel member of the phylum Chlamydiae from the broad nosed pipefish, Syngnathus typhle.PloS one · 2013Article
- Lack of effective anti-apoptotic activities restricts growth of Parachlamydiaceae in insect cells.PloS one · 2012Article
- mathFISH, a web tool that uses thermodynamics-based mathematical models for in silico evaluation of oligonucleotide probes for fluorescence in situ hybridization.Applied and environmental microbiology · 2011Article
- Two types of endosymbiotic bacteria in the enigmatic marine worm Xenoturbella bocki.Applied and environmental microbiology · 2010Article
- Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization.Journal of clinical microbiology · 2007Article
- probeBase--an online resource for rRNA-targeted oligonucleotide probes: new features 2007.Nucleic acids research · 2007Article
- Chlamydia pneumoniae replicates in Kupffer cells in mouse model of liver infection.World journal of gastroenterology · 2006Article
- Pathogenic potential of novel Chlamydiae and diagnostic approaches to infections due to these obligate intracellular bacteria.Clinical microbiology reviews · 2006Review
- Rapid diagnosis of bacterial meningitis by real-time PCR and fluorescence in situ hybridization.Journal of clinical microbiology · 2005Article
- Effects of a probiotic strain of Enterococcus faecium on the rate of natural chlamydia infection in swine.Infection and immunity · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydiae are important pathogens of humans and animals but diagnosis of chlamydial infections is still hampered by inadequate detection methods. Fluorescence in situ hybridization (FISH) using rRNA-targeted oligonucleotide probes is widely used for the investigation of uncultured bacteria in complex microbial communities and has recently also been shown to be a valuable tool for the rapid detection of various bacterial pathogens in clinical specimens. Here we report on the development and evaluation of a hierarchic probe set for the specific detection and differentiation of chlamydiae, particularly C. pneumoniae, C. trachomatis, C. psittaci, and the recently described chlamydia-like bacteria comprising the novel genera Neochlamydia and PARACHLAMYDIA: The specificity of the nine newly developed probes was successfully demonstrated by in situ hybridization of experimentally infected amoebae and HeLa 229 cells, including HeLa 229 cells coinfected with C. pneumoniae and C. trachomatis. FISH reliably stained chlamydial inclusions as early as 12 h postinfection. The sensitivity of FISH was further confirmed by combination with direct fluorescence antibody staining. In contrast to previously established detection methods for chlamydiae, FISH was not susceptible to false-positive results and allows the detection of all recognized chlamydiae in one single step.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.