ArticleApplied and environmental microbiology2002
Nonfunctionality of Aspergillus sojae aflR in a strain of Aspergillus parasiticus with a disrupted aflR gene.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Population Genomics of Aspergillus sojae is Shaped by the Food Environment.Genome biology and evolution · 2025Article
- Are CurrentMycobiology · 2023Article
- Koji Starter and Koji World in Japan.Journal of fungi (Basel, Switzerland) · 2021Review
- The Transcriptional Regulator HbxA Governs Development, Secondary Metabolism, and Virulence in Aspergillus fumigatus.Applied and environmental microbiology · 2020Article
- Review
- Review
- Heterologous Production of a Novel Cyclic Peptide Compound, KK-1, inFrontiers in microbiology · 2018Article
- Expression of ustR and the Golgi protease KexB are required for ustiloxin B biosynthesis in Aspergillus oryzae.AMB Express · 2016Article
- Article
- Functional expression and subcellular localization of the aflatoxin pathway enzyme Ver-1 fused to enhanced green fluorescent protein.Applied and environmental microbiology · 2008Article
- Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future.DNA research : an international journal for rapid publication of reports on genes and genomes · 2008Review
- What can comparative genomics tell us about species concepts in the genus Aspergillus?Studies in mycology · 2007Article
- Understanding the genetics of regulation of aflatoxin production and Aspergillus flavus development.Mycopathologia · 2006Review
- Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae.Molecular genetics and genomics : MGG · 2006Article
- Molecular analysis of an inactive aflatoxin biosynthesis gene cluster in Aspergillus oryzae RIB strains.Applied and environmental microbiology · 2006Article
- Efficient gene disruption in the koji-mold Aspergillus sojae using a novel variation of the positive-negative method.Molecular genetics and genomics : MGG · 2004Article
- Clustered pathway genes in aflatoxin biosynthesis.Applied and environmental microbiology · 2004Review
- Mycotoxins.Clinical microbiology reviews · 2003Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspergillus sojae belongs to the Aspergillus section Flavi but does not produce aflatoxins. The functionality of the A. sojae aflR gene (aflRs) was examined by transforming it into an DeltaaflR strain of A. parasiticus, derived from a nitrate-nonutilizing, versicolorin A (VERA)-accumulating strain. The A. parasiticus aflR gene (aflRp) transformants produced VERA, but the aflRs transformants did not. Even when aflRs was placed under the control of the amylase gene (amyB) promoter of Aspergillus oryzae, the amy(p)::aflRs transformants did not produce VERA. A chimeric construct containing the aflRs promoter plus the aflRs N- and aflRp C-terminal coding regions could restore VERA production, but a construct containing the aflRp promoter plus the aflRp N- and aflRs C-terminal coding regions could not. These results show that the A. sojae aflR promoter is functional in A. parasiticus and that the HAHA motif does not affect the function of the resulting hybrid AflR. We conclude that the lack of aflatoxin production by A. sojae can be attributed, at least partially, to the premature termination defect in aflRs, which deletes the C-terminal transcription activation domain that is critical for the expression of aflatoxin biosynthetic genes.
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