ArticleThe EMBO journal2002
Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
- Interactions between the transcription factors FfmA and AtrR are required to properly regulate gene expression in the fungus Aspergillus fumigatus.G3 (Bethesda, Md.) · 2023Article
- Transcription factor FfmA interacts both physically and genetically with AtrR to properly regulate gene expression in the fungusbioRxiv : the preprint server for biology · 2023Article
- Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1.Current genetics · 2023Article
- Reengineering of 7-dehydrocholesterol biosynthesis inFrontiers in microbiology · 2022Article
- Article
- Regulation of Ergosterol Biosynthesis inGenes · 2020Review
- Lipid Exchangers: Cellular Functions and Mechanistic Links With Phosphoinositide Metabolism.Frontiers in cell and developmental biology · 2020Review
- Oxygen-responsive transcriptional regulation of lipid homeostasis in fungi: Implications for anti-fungal drug development.Seminars in cell & developmental biology · 2018Review
- Common and divergent features of galactose-1-phosphate and fructose-1-phosphate toxicity in yeast.Molecular biology of the cell · 2018Article
- The Influence of Polyploidy on the Evolution of Yeast Grown in a Sub-Optimal Carbon Source.Molecular biology and evolution · 2017Article
- Binding to RNA regulates Set1 function.Cell discovery · 2017Article
- Expression of C-5 sterol desaturase from an edible mushroom in fisson yeast enhances its ethanol and thermotolerance.PloS one · 2017Article
- Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p.Nature communications · 2015Article
- Novel antifungal drug discovery based on targeting pathways regulating the fungus-conserved Upc2 transcription factor.Antimicrobial agents and chemotherapy · 2014Article
- Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae.Eukaryotic cell · 2013Article
- Physical and Genetic Interactions Between Uls1 and the Slx5-Slx8 SUMO-Targeted Ubiquitin Ligase.G3 (Bethesda, Md.) · 2013Article
- Genetic and genomic architecture of the evolution of resistance to antifungal drug combinations.PLoS genetics · 2013Article
- Article
- Amyloids and yeast prion biology.Biochemistry · 2013Article
- Systematic measurement of transcription factor-DNA interactions by targeted mass spectrometry identifies candidate gene regulatory proteins.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae MOT3 gene encodes a nuclear protein implicated in both repression and activation of transcription. However, a mot3 Delta mutation causes only mild phenotypes under normal growth conditions. To learn more about Mot3 function, we have performed a synthetic lethal screen. This screen identified PAN1, a gene required for normal endocytosis, and VPS41, a gene required for vacuolar fusion and protein targeting, suggesting a role for Mot3 in the regulation of membrane-related genes. Transcriptional analyses show that Mot3 represses transcription of ERG2, ERG6 and ERG9, genes required for ergosterol biosynthesis, during both aerobic and hypoxic growth. Chromatin immunoprecipitation experiments suggest that this repression is direct. Ergosterol has been shown to be required for endocytosis and homotypic vacuole fusion, providing a link between Mot3 and these processes. Consistent with these results, mot3 Delta mutants have a number of related defects, including impaired homotypic vacuole fusion and increased sterol levels. Taken together, our data suggest that proper transcriptional regulation of ergosterol biosynthetic genes by Mot3 is important for normal vacuolar function and probably for the endocytic membrane transport system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.