ArticleThe EMBO journal2002
The Rho-GAP Bem2p plays a GAP-independent role in the morphogenesis checkpoint.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Amino acid substitutions in specific proteins correlate with farnesol unresponsiveness in Candida albicans.BMC genomics · 2023Article
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- The mitotic exit network regulates the spatiotemporal activity of Cdc42 to maintain cell size.The Journal of cell biology · 2021Article
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- Haspin regulates Ras localization to promote Cdc24-driven mitotic depolarization.Cell discovery · 2020Article
- Haspin Modulates the G2/M Transition Delay in Response to Polarization Failures in Budding Yeast.Frontiers in cell and developmental biology · 2020Article
- Article
- Parallel Actin-Independent Recycling Pathways Polarize Cdc42 in Budding Yeast.Current biology : CB · 2016Article
- Rho1- and Pkc1-dependent phosphorylation of the F-BAR protein Syp1 contributes to septin ring assembly.Molecular biology of the cell · 2015Article
- The GTPase-activating protein Rga1 interacts with Rho3 GTPase and may regulate its function in polarized growth in budding yeast.PloS one · 2015Article
- Inhibition of Cdc42 during mitotic exit is required for cytokinesis.The Journal of cell biology · 2013Article
- Control of polarized growth by the Rho family GTPase Rho4 in budding yeast: requirement of the N-terminal extension of Rho4 and regulation by the Rho GTPase-activating protein Bem2.Eukaryotic cell · 2013Article
- Polarization of Diploid Daughter Cells Directed by Spatial Cues and GTP Hydrolysis of Cdc42 in Budding YeastPloS one · 2013Article
- GDI-mediated cell polarization in yeast provides precise spatial and temporal control of Cdc42 signaling.PLoS computational biology · 2013Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- Morphogenesis and the cell cycle.Genetics · 2012Review
- The Rho1 GTPase-activating protein CgBem2 is required for survival of azole stress in Candida glabrata.The Journal of biological chemistry · 2011Article
- Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in maintaining TOR complex 2 integrity and coupling to downstream signaling.Eukaryotic cell · 2008Article
- Yeast and fungal morphogenesis from an evolutionary perspective.Seminars in cell & developmental biology · 2008Review
- Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence.The EMBO journal · 2007Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae morphogenesis checkpoint delays mitosis in response to insults that impair actin organization and/or bud formation. The delay is due to accumulation of the inhibitory kinase Swe1p, which phosphorylates the cyclin-dependent kinase Cdc28p. Having screened through a panel of yeast mutants with defects in cell morphogenesis, we report here that the polarity establishment protein Bem2p is required for the checkpoint response. Bem2p is a Rho-GTPase activating protein (GAP) previously shown to act on Rho1p, and we now show that it also acts on Cdc42p, the GTPase primarily responsible for establishment of cell polarity in yeast. Whereas the morphogenesis role of Bem2p required GAP activity, the checkpoint role of Bem2p did not. Instead, this function required an N-terminal Bem2p domain. Thus, this single protein has a GAP-dependent role in promoting cell polarity and a GAP-independent role in responding to defects in cell polarity by enacting the checkpoint. Surprisingly, Swe1p accumulation occurred normally in bem2 cells, but they were nevertheless unable to promote Cdc28p phosphorylation. Therefore, Bem2p defines a novel pathway in the morphogenesis checkpoint.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.