ArticleThe EMBO journal2002
Calmodulin and lipid binding to synaptobrevin regulates calcium-dependent exocytosis.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 118 citations in OpenAlex.
- CaScience advances · 2025Article
- Sphingosylphosphorylcholine alleviates pressure overload-induced myocardial remodeling in mice via inhibiting CaM-JNK/p38 signaling pathway.Acta pharmacologica Sinica · 2024Article
- Endosomal trafficking of two-pore KeLife · 2023Article
- The Role of Calmodulin vs. Synaptotagmin in Exocytosis.Frontiers in molecular neuroscience · 2021Review
- Tetanus insensitive VAMP2 differentially restores synaptic and dense core vesicle fusion in tetanus neurotoxin treated neurons.Scientific reports · 2020Article
- Interplay between morphogen-directed positional information systems and physiological signaling.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Review
- Retrograde trafficking and quality control of yeast synaptobrevin, Snc1, are conferred by its transmembrane domain.Molecular biology of the cell · 2019Article
- Copine-6 Binds to SNAREs and Selectively Suppresses Spontaneous Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- v-SNARE function in chromaffin cells.Pflugers Archiv : European journal of physiology · 2018Review
- Platelet signaling: a complex interplay between inhibitory and activatory networks.Journal of thrombosis and haemostasis : JTH · 2016Review
- Morphine Antidependence ofEvidence-based complementary and alternative medicine : eCAM · 2016Article
- Phosphatidylserine-Dependent Catalysis of Stalk and Pore Formation by Synaptobrevin JMR-TMD Peptide.Biophysical journal · 2015Article
- Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and β cells.Nature communications · 2015Article
- Characterization of P4 ATPase Phospholipid Translocases (Flippases) in Human and Rat Pancreatic Beta Cells: THEIR GENE SILENCING INHIBITS INSULIN SECRETION.The Journal of biological chemistry · 2015Article
- SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors.Molecular biology of the cell · 2015Article
- Characterization of phospholipids in insulin secretory granules and mitochondria in pancreatic beta cells and their changes with glucose stimulation.The Journal of biological chemistry · 2015Article
- Juxtamembrane tryptophans of synaptobrevin 2 control the process of membrane fusion.FEBS letters · 2013Article
- Annexin A7 and SNAP23 interactions in alveolar type II cells and in vitro: a role for Ca(2+) and PKC.Biochimica et biophysica acta · 2012Article
- Role of PI(4,5)P(2) in vesicle exocytosis and membrane fusion.Sub-cellular biochemistry · 2012Review
- Secretagogues of lung surfactant increase annexin A7 localization with ABCA3 in alveolar type II cells.Biochimica et biophysica acta · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurotransmitter release involves the assembly of a heterotrimeric SNARE complex composed of the vesicle protein synaptobrevin (VAMP 2) and two plasma membrane partners, syntaxin 1 and SNAP-25. Calcium influx is thought to control this process via Ca(2+)-binding proteins that associate with components of the SNARE complex. Ca(2+)/calmodulin or phospholipids bind in a mutually exclusive fashion to a C-terminal domain of VAMP (VAMP(77-90)), and residues involved were identified by plasmon resonance spectroscopy. Microinjection of wild-type VAMP(77-90), but not mutant peptides, inhibited catecholamine release from chromaffin cells monitored by carbon fibre amperometry. Pre-incubation of PC12 pheochromocytoma cells with the irreversible calmodulin antagonist ophiobolin A inhibited Ca(2+)-dependent human growth hormone release in a permeabilized cell assay. Treatment of permeabilized cells with tetanus toxin light chain (TeNT) also suppressed secretion. In the presence of TeNT, exocytosis was restored by transfection of TeNT-resistant (Q(76)V, F(77)W) VAMP, but additional targeted mutations in VAMP(77-90) abolished its ability to rescue release. The calmodulin- and phospholipid-binding domain of VAMP 2 is thus required for Ca(2+)-dependent exocytosis, possibly to regulate SNARE complex assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.