ArticleJournal of bacteriology2002
Suppressive subtractive hybridization detects extensive genomic diversity in Thermotoga maritima.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 68 citations in OpenAlex.
- MOBHunter: a data integration platform for identification and classification of mobile genetic elements in microbial genomes.Nucleic acids research · 2025Article
- Novel, Deep-Branching Heterotrophic Bacterial Populations Recovered from Thermal Spring Metagenomes.Frontiers in microbiology · 2016Article
- Horizontal gene transfer in osmotrophs: playing with public goods.Nature reviews. Microbiology · 2013Article
- Hyperthermophilic Thermotoga species differ with respect to specific carbohydrate transporters and glycoside hydrolases.Applied and environmental microbiology · 2012Article
- Genome sequence of Thermotoga sp. strain RQ2, a hyperthermophilic bacterium isolated from a geothermally heated region of the seafloor near Ribeira Quente, the Azores.Journal of bacteriology · 2011Article
- Quantification of Propionibacterium acidipropionici P169 bacteria in environmental samples by use of strain-specific primers derived by suppressive subtractive hybridization.Applied and environmental microbiology · 2011Article
- The genus Thermotoga: recent developments.Environmental technology · 2010Article
- On the chimeric nature, thermophilic origin, and phylogenetic placement of the Thermotogales.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Extensive genome rearrangements and multiple horizontal gene transfers in a population of pyrococcus isolates from Vulcano Island, Italy.Applied and environmental microbiology · 2008Article
- Genomic differences between Fibrobacter succinogenes S85 and Fibrobacter intestinalis DR7, identified by suppression subtractive hybridization.Applied and environmental microbiology · 2008Article
- Evolution of mal ABC transporter operons in the Thermococcales and Thermotogales.BMC evolutionary biology · 2008Article
- Genomic diversity in Staphylococcus xylosus.Applied and environmental microbiology · 2007Article
- Systematic overestimation of gene gain through false diagnosis of gene absence.Genome biology · 2007Article
- Construction and characterization of a highly redundant Pseudomonas aeruginosa genomic library prepared from 12 clinical isolates: application to studies of gene distribution among populations.International journal of pediatric otorhinolaryngology · 2006Article
- Colocation of genes encoding a tRNA-mRNA hybrid and a putative signaling peptide on complementary strands in the genome of the hyperthermophilic bacterium Thermotoga maritima.Journal of bacteriology · 2006Article
- Chromosome evolution in the Thermotogales: large-scale inversions and strain diversification of CRISPR sequences.Journal of bacteriology · 2006Article
- Article
- Several archaeal homologs of putative oligopeptide-binding proteins encoded by Thermotoga maritima bind sugars.Applied and environmental microbiology · 2006Article
- An expression-driven approach to the prediction of carbohydrate transport and utilization regulons in the hyperthermophilic bacterium Thermotoga maritima.Journal of bacteriology · 2005Article
- Gene transfer and genome plasticity in Thermotoga maritima, a model hyperthermophilic species.Journal of bacteriology · 2005Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comparisons between genomes of closely related bacteria often show large variations in gene content, even between strains of the same species. Such studies have focused mainly on pathogens; here, we examined Thermotoga maritima, a free-living hyperthermophilic bacterium, by using suppressive subtractive hybridization. The genome sequence of T. maritima MSB8 is available, and DNA from this strain served as a reference to obtain strain-specific sequences from Thermotoga sp. strain RQ2, a very close relative (approximately 96% identity for orthologous protein-coding genes, 99.7% identity in the small-subunit rRNA sequence). Four hundred twenty-six RQ2 subtractive clones were sequenced. One hundred sixty-six had no DNA match in the MSB8 genome. These differential clones comprise, in sum, 48 kb of RQ2-specific DNA and match 72 genes in the GenBank database. From the number of identical clones, we estimated that RQ2 contains 350 to 400 genes not found in MSB8. Assuming a similar genome size, this corresponds to 20% of the RQ2 genome. A large proportion of the RQ2-specific genes were predicted to be involved in sugar transport and polysaccharide degradation, suggesting that polysaccharides are more important as nutrients for this strain than for MSB8. Several clones encode proteins involved in the production of surface polysaccharides. RQ2 encodes multiple subunits of a V-type ATPase, while MSB8 possesses only an F-type ATPase. Moreover, an RQ2-specific MutS homolog was found among the subtractive clones and appears to belong to a third novel archaeal type MutS lineage. Southern blot analyses showed that some of the RQ2 differential sequences are found in some other members of the order Thermotogales, but the distribution of these variable genes is patchy, suggesting frequent lateral gene transfer within the group.
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