ArticleJournal of bacteriology2002
The nptA gene of Vibrio cholerae encodes a functional sodium-dependent phosphate cotransporter homologous to the type II cotransporters of eukaryotes.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Phosphate deprivation restricts bacterial degradation of the marine polysaccharide fucoidan.Nature microbiology · 2026Article
- Multiple clades of regulators contribute to bacterial phosphate homeostasis and pathogenesis.bioRxiv : the preprint server for biology · 2025Article
- Article
- Adaptive Responses of Cyanobacteria to Phosphate Limitation: A Focus on Marine Diazotrophs.Environmental microbiology · 2024Review
- Effects of NaCl Concentration on the Behavior ofFoods (Basel, Switzerland) · 2022Article
- Coordination of Phosphate and Magnesium Metabolism in Bacteria.Advances in experimental medicine and biology · 2022Article
- Accumulation of ambient phosphate into the periplasm of marine bacteria is proton motive force dependent.Nature communications · 2020Article
- PhoPR Contributes to Staphylococcus aureus Growth during Phosphate Starvation and Pathogenesis in an Environment-Specific Manner.Infection and immunity · 2018Article
- Acquisition of the Phosphate Transporter NptA Enhances Staphylococcus aureus Pathogenesis by Improving Phosphate Uptake in Divergent Environments.Infection and immunity · 2018Article
- Predicting Species-Resolved Macronutrient Acquisition during Succession in a Model Phototrophic Biofilm Using an Integrated 'Omics Approach.Frontiers in microbiology · 2017Article
- Physiological Roles of the Dual Phosphate Transporter Systems in Low and High Phosphate Conditions and in Capsule Maintenance of Streptococcus pneumoniae D39.Frontiers in cellular and infection microbiology · 2016Article
- Differential expression of a sodium-phosphate cotransporter among Vibrio vulnificus strains.Microbial ecology · 2014Article
- Transposon mutagenesis identified chromosomal and plasmid genes essential for adaptation of the marine bacterium Dinoroseobacter shibae to anaerobic conditions.Journal of bacteriology · 2013Article
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Authors and funding
5 authors.
Funding
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Abstract
The nptA gene of Vibrio cholerae has significant protein sequence homology with type II sodium-dependent phosphate (P(i)) cotransporters found in animals but not previously identified in prokaryotes. The phylogeny of known type II cotransporter sequences indicates that nptA may be either an ancestral gene or a gene acquired from a higher eukaryotic source. The gene was cloned into an expression vector under the control of an inducible promoter and expressed in Escherichia coli. The results demonstrate that nptA encodes a functional protein with activity similar to that of the animal enzyme, catalyzing high-affinity, sodium-dependent P(i) uptake with comparable affinities for both sodium and phosphate ions. Furthermore, the activity of NptA is influenced by pH, again in a manner similar to that of the NaPi-2a subtype of the animal enzyme, although it lacks the corresponding REK motif thought to be responsible for this phenomenon. P(i) uptake activity, a component of which appeared to be sodium dependent, was increased in V. cholerae by phosphate starvation. However, it appears from the use of a reporter gene expressed from the nptA promoter that none of this activity is attributable to the induction of expression from nptA. It is thus proposed that the physiological function of NptA protein may be the rapid uptake of P(i) in preparation for rapid growth in nutrient-rich environments and that it may therefore play a role in establishing infection.
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