ArticleNucleic acids research2002
Analysis of the role of Caenorhabditis elegans GC-AG introns in regulated splicing.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- An in vivo parallelized reporter assay to uncover tissue-specific splicing regulatory sequences in a multicellular animal.Nucleic acids research · 2026Article
- Difference Analysis Among Six Kinds of Acceptor Splicing Sequences by the Dispersion Features of 6-mer Subsets in Human Genes.Biology · 2025Article
- Comprehensive database and evolutionary dynamics of U12-type introns.Nucleic acids research · 2020Article
- mRNA Editing, Processing and Quality Control inGenetics · 2020Review
- GC-AG Introns Features in Long Non-coding and Protein-Coding Genes Suggest Their Role in Gene Expression Regulation.Frontiers in genetics · 2020Article
- The main WAP isoform usually found in camel milk arises from the usage of an improbable intron cryptic splice site in the precursor to mRNA in which a GC-AG intron occurs.BMC genetics · 2019Article
- Functional Variation of Two Novel Cellulases,International journal of molecular sciences · 2018Article
- Validation of Splicing Events in Transcriptome Sequencing Data.International journal of molecular sciences · 2017Article
- The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence.Genome biology · 2016Article
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- Deciphering the genomic structure, function and evolution of carotenogenesis related phytoene synthases in grasses.BMC genomics · 2012Article
- Origin and evolution of spliceosomal introns.Biology direct · 2012Review
- Short-read sequencing for genomic analysis of the brown rot fungus Fibroporia radiculosa.Applied and environmental microbiology · 2012Article
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- Cloning and characterization of squalene synthase gene from Fusarium fujikuroi (Saw.) Wr.Journal of industrial microbiology & biotechnology · 2010Article
- Article
- Genome-wide mapping of alternative splicing in Arabidopsis thaliana.Genome research · 2010Article
- Oscheius tipulae as an example of eEF1A gene diversity in nematodes.Journal of molecular evolution · 2008Article
- Accumulation of GC donor splice signals in mammals.Biology direct · 2008Article
- STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo.Genes & development · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
GC-AG introns represent 0.7% of total human pre-mRNA introns. To study the function of GC-AG introns in splicing regulation, 196 cDNA-confirmed GC-AG introns were identified in Caenorhabditis elegans. These represent 0.6% of the cDNA- confirmed intron data set for this organism. Eleven of these GC-AG introns are involved in alternative splicing. In a comparison of the genomic sequences of homologous genes between C.elegans and Caenorhabditis briggsae for 26 GC-AG introns, the C at the +2 position is conserved in only five of these introns. A system to experimentally test the function of GC-AG introns in alternative splicing was developed. Results from these experiments indicate that the conserved C at the +2 position of the tenth intron of the let-2 gene is essential for developmentally regulated alternative splicing. This C allows the splice donor to function as a very weak splice site that works in balance with an alternative GT splice donor. A weak GT splice donor can functionally replace the GC splice donor and allow for splicing regulation. These results indicate that while the majority of GC-AG introns appear to be constitutively spliced and have no evolutionary constraints to prevent them from being GT-AG introns, a subset of GC-AG introns is involved in alternative splicing and the C at the +2 position of these introns can have an important role in splicing regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.