ArticleNucleic acids research2002
Detection and analysis of hairpin II, an essential metastable structural element in viroid replication intermediates.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Viroids, Satellite RNAs and Prions: Folding of Nucleic Acids and Misfolding of Proteins.Viruses · 2024Article
- Predicting the Structure of a Viroid : Structure, Structure Distribution, Consensus Structure, and Structure Drawing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Viroid research and its significance for RNA technology and basic biochemistry.Nucleic acids research · 2018Review
- Viroids: from genotype to phenotype just relying on RNA sequence and structural motifs.Frontiers in microbiology · 2012Article
- The RNA strands of the plus and minus polarities of peach latent mosaic viroid fold into different structures.RNA (New York, N.Y.) · 2010Article
- An artificial chimeric derivative of Citrus viroid V involves the terminal left domain in pathogenicity.Molecular plant pathology · 2009Article
- A genomic map of viroid RNA motifs critical for replication and systemic trafficking.The Plant cell · 2008Article
- Evidence for the existence of the loop E motif of Potato spindle tuber viroid in vivo.Journal of virology · 2007Article
- Tertiary structural and functional analyses of a viroid RNA motif by isostericity matrix and mutagenesis reveal its essential role in replication.Journal of virology · 2006Article
- Inhibition of cell growth and shoot development by a specific nucleotide sequence in a noncoding viroid RNA.The Plant cell · 2003Article
- A mini-RNA containing the tetraloop, wobble-pair and loop E motifs of the central conserved region of potato spindle tuber viroid is processed into a minicircle.Nucleic acids research · 2003Article
- A new structural motif in the left terminal domain of large viroids identified by covariation analysis.Virus genes · 2003Article
- RNA conformational changes in the life cycles of RNA viruses, viroids, and virus-associated RNAs.Biochimica et biophysica actaReview
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2 authors.
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Abstract
In (-)-stranded replication intermediates of the potato spindle tuber viroid (PSTVd) a thermodynamically metastable structure containing a specific hairpin structure (HP II) has been proposed to be essential for viroid replication. In the present work a method was devised allowing the direct detection of the HP II structure in vitro and in vivo using a biophysical approach. An RNA oligonucleotide was constructed which specifically binds to the HP II loop region in transient (-)-strand intermediates. Analysis of the resulting oligonucleotide/HP II complexes on temperature-gradient gels enabled us to follow the formation of HP II during in vitro transcription by T7 RNA polymerase. Moreover, we were able to demonstrate the formation of HP II during viroid replication in potato (Solanum tuberosum) cells.
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