ArticleNucleic acids research2002
High frequencies of Minos transposon mobilization are obtained in insects by using in vitro synthesized mRNA as a source of transposase.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Targeting the cell membrane in established and emerging model organisms.bioRxiv : the preprint server for biology · 2025Article
- Article
- Article
- Highly efficient integration and expression of piggyBac-derived cassettes in the honeybee (Apis mellifera).Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Germ line transformation of the silkworm, Bombyx mori, using the transposable element Minos.Molecular genetics and genomics : MGG · 2007Article
- Stable expression of human growth hormone over 50 generations in transgenic insect larvae.Transgenic research · 2007Article
- Site-directed transposon integration in human cells.Nucleic acids research · 2007Article
- Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives.Genome biology · 2007Review
- The DNA transposon Minos as a tool for transgenesis and functional genomic analysis in vertebrates and invertebrates.Genome biology · 2007Review
- Article
- Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- High-level gene expression in Aedes albopictus cells using a baculovirus Hr3 enhancer and IE1 transactivator.BMC molecular biology · 2004Article
- Article
- Article
- Construction of synthetic constitutive promoters and evaluation of their expression strengths in Sf9, HEK293T, and BmN cells.Science progressArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the most frequently encountered problems in transposon-mediated transgenesis is low transformation frequency, often resulting from difficulty in expressing from injected plasmid DNA constructs adequate levels of transposase in embryos. Capped RNA corresponding to the spliced transcript of the Minos transposable element has been synthesized in vitro and shown to be an effective source of transposase protein for Minos transposon mobilization. Transposase produced by this mRNA is shown to catalyze excision of a Minos transposon from plasmid DNA in Medfly embryos. When injected into Drosophila or Medfly embryos, transposase mRNA leads to a several-fold increase in transformation efficiencies compared with injected plasmids expressing transposase. Also, frequent mobilization of a Minos transposon from the X chromosome into autosomes was demonstrated after injections of Minos transposase mRNA into pre-blastoderm Drosophila embryos. The high rates of transposition achieved with transposase mRNA suggest that this is a powerful system for genetic applications in Drosophila and other insects.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.