ArticleBMC microbiology2002
Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2).
Article in BMC microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
- A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- In vitro antifungal resistance profile of Candida strains isolated from Saudi women suffering from vulvovaginitis.European journal of medical research · 2020Article
- Evaluation of Fluorescent Capillary Electrophoresis for Rapid Identification of Candida Fungal Infections.Journal of clinical microbiology · 2016Article
- Article
- A rapid and easy method for the DNA extraction from Cryptococcus neoformans.Biological procedures online · 2011Article
- Diagnosis of invasive fungal infections by a real-time panfungal PCR assay in immunocompromised pediatric patients.Leukemia · 2010Article
- Molecular analysis of Candida albicans isolates from clinical specimens.Mycopathologia · 2010Article
- Identification of fungal species by fragment length analysis of the internally transcribed spacer 2 region.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Microflora of the penile skin-lined neovagina of transsexual women.BMC microbiology · 2009Article
- Species-specific identification of a wide range of clinically relevant fungal pathogens by use of Luminex xMAP technology.Journal of clinical microbiology · 2009Article
- Identification of medically important Candida and non-Candida yeast species by an oligonucleotide array.Journal of clinical microbiology · 2007Article
- Effect of iron on the expression of sirR and sitABC in biofilm-associated Staphylococcus epidermidis.BMC microbiology · 2006Article
- Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions.Journal of clinical microbiology · 2006Article
- Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species.Journal of clinical microbiology · 2005Article
- Cryptococcus gattii in AIDS patients, southern California.Emerging infectious diseases · 2005Article
- Susceptibility patterns and molecular identification of Trichosporon species.Antimicrobial agents and chemotherapy · 2005Article
- Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses.Journal of clinical microbiology · 2005Article
- Multilocus DNA sequence comparisons rapidly identify pathogenic molds.Journal of clinical microbiology · 2005Article
- An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems.BMC microbiology · 2005Article
- Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species.Journal of clinical microbiology · 2004Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe number of patients candidate to yeast infection has increased during the last years. Also the variety of species of clinical importance has increased. Correct species identification is often important for efficient therapy, but is based on phenotypic features and is sometimes time-consuming and depends largely on the expertise of technicians. Therefore, we evaluated the feasability of PCR-based amplification of the Internally Transcribed Spacer region 2, followed by fragment size analysis on the ABI Prism 310 for the identification of clinically important yeasts.
resultsA rapid DNA-extraction method, based on simple boiling freezing was introduced. Of the 25 species tested, 22 could be identified unambiguously by scoring the length of the ITS2-region. No distinction could be made between the species T. asteroides and T. inkin and between T. mucoides and T. ovoides. The two varieties of Cryptococcus neoformans (var. neoformans and var. gattii) could be differentiated from each other due to a one bp length difference of the ITS2 fragment. The three C. laurentii isolates were split in two groups according to their ITS2-fragment lengths, in correspondence with the phylogenetic groups described previously. Since the obtained fragment lenghts compared well to those described previously, an internationally usable library of ITS2 fragment lengths can be constructed.
conclusionsThe existing ITS2 size based library enables identification of most of the clinically important yeast species, within 6 hours starting from a single colony, can be easily updated when new species are described. Data can be exchanged between laboratories.
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