ArticleMolecular and cellular biology2002
Role of the p68 subunit of human DNA polymerase alpha-primase in simian virus 40 DNA replication.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- The human papillomavirus DNA helicase E1 binds, stimulates, and confers processivity to cellular DNA polymerase epsilon.Nucleic acids research · 2018Article
- Modeling of the SV40 DNA Replication Machine.Genes · 2012Article
- Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase α-primase.The Journal of biological chemistry · 2012Article
- A specific docking site for DNA polymerase {alpha}-primase on the SV40 helicase is required for viral primosome activity, but helicase activity is dispensable.The Journal of biological chemistry · 2010Article
- Structure of a DNA polymerase alpha-primase domain that docks on the SV40 helicase and activates the viral primosome.The Journal of biological chemistry · 2010Article
- Aberrant DNA polymerase alpha is excluded from the nucleus by defective import and degradation in the nucleus.The Journal of biological chemistry · 2009Article
- Physical interactions between Mcm10, DNA, and DNA polymerase alpha.The Journal of biological chemistry · 2009Article
- Characterization of RNA primers synthesized by the human breast cancer cell DNA synthesome.Journal of cellular biochemistry · 2009Article
- SV40 DNA replication: from the A gene to a nanomachine.Virology · 2009Review
- Domain architecture and biochemical characterization of vertebrate Mcm10.The Journal of biological chemistry · 2008Article
- Protein encoded by HSV-1 stimulation-related gene 1 (HSRG1) interacts with and inhibits SV40 large T antigen.Cell proliferation · 2006Article
- The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.Molecular and cellular biology · 2004Article
- DNA replication: a complex matter.EMBO reports · 2003Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
DNA polymerase alpha-primase (pol-prim) is a heterotetramer with DNA polymerase and primase activities. The polymerase (p180) and primase (p48 and p58) subunits synthesize primers and extend them, but the function of the remaining subunit (p68) is poorly understood. Genetic studies in yeast suggested an essential role for the p68 ortholog in early S phase prior to the hydroxyurea-sensitive step, possibly a regulatory role in initiation of DNA replication, but found no evidence for an essential function of p68 later in S phase. To investigate whether the human p68 subunit has an essential role in DNA replication, we examined the ability of a purified trimeric human pol-prim lacking p68 to initiate simian virus 40 DNA replication in vitro and to synthesize and elongate primers on single-stranded DNA in the presence of T antigen and replication protein A (RPA). Both activities of trimeric pol-prim were defective, but activity was recovered upon addition of separately purified p68. Phosphorylation of p68 by cyclin A-dependent protein kinase also inhibited both activities of pol-prim. The data strongly suggest that the p68 subunit is required for priming activity of pol-prim in the presence of RPA and T antigen, both during initiation at the origin and during lagging strand replication.
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