ArticleNucleic acids research2002
Real-time monitoring of rolling-circle amplification using a modified molecular beacon design.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Design and Optimization of Isothermal Gene Amplification for Generation of High-Gain Oligonucleotide Products by MicroRNAs.ACS measurement science au · 2024Article
- Single-copy detection of somatic variants from solid and liquid biopsy.Scientific reports · 2021Article
- Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods.Frontiers in molecular biosciences · 2021Review
- Laboratory testing of SARS-CoV, MERS-CoV, and SARS-CoV-2 (2019-nCoV): Current status, challenges, and countermeasures.Reviews in medical virology · 2020Review
- Integration of microbead DNA handling with optomagnetic detection in rolling circle amplification assays.Mikrochimica acta · 2019Article
- Simultaneous Detection of Different Zika Virus Lineages via Molecular Computation in a Point-of-Care Assay.Viruses · 2018Article
- Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing.Pharmaceuticals (Basel, Switzerland) · 2018Review
- Interlinked DNA nano-circles for measuring topoisomerase II activity at the level of single decatenation events.Nucleic acids research · 2017Article
- Optimized Detection of Plasmodium falciparum Topoisomerase I Enzyme Activity in a Complex Biological Sample by the Use of Molecular Beacons.Sensors (Basel, Switzerland) · 2016Article
- Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP.Nucleic acids research · 2016Article
- Assessment for Melting Temperature Measurement of Nucleic Acid by HRM.Journal of analytical methods in chemistry · 2016Article
- Rationally designed molecular beacons for bioanalytical and biomedical applications.Chemical Society reviews · 2015Review
- Article
- Real-time detection of isothermal amplification reactions with thermostable catalytic hairpin assembly.Journal of the American Chemical Society · 2013Article
- Development of a panel of recombinase polymerase amplification assays for detection of biothreat agents.Journal of clinical microbiology · 2013Article
- Recombinase polymerase amplification assay for rapid detection of Francisella tularensis.Journal of clinical microbiology · 2012Article
- Amplified microRNA detection by templated chemistry.Nucleic acids research · 2012Article
- Rapid identification of bio-molecules applied for detection of biosecurity agents using rolling circle amplification.PloS one · 2012Article
- Glycosylases and AP-cleaving enzymes as a general tool for probe-directed cleavage of ssDNA targets.Nucleic acids research · 2010Article
- The method of single-nucleotide variations detection using capillary electrophoresis and molecular beacons.Molecular biology reports · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a method to monitor rolling-circle replication of circular oligonucleotides in dual-color and in real-time using molecular beacons. The method can be used to study the kinetics of the polymerization reaction and to amplify and quantify circularized oligonucleotide probes in a rolling-circle amplification (RCA) reaction. Modified molecular beacons were made of 2'-O-Me-RNA to prevent 3' exonucleolytic degradation by the polymerase used. Moreover, the complement of one of the stem sequences of the molecular beacon was included in the RCA products to avoid fluorescence quenching due to inter-molecular hybridization of neighboring molecular beacons hybridizing to the concatemeric polymerization product. The method allows highly accurate quantification of circularized DNA over a broad concentration range by relating the signal from the test DNA circle to an internal reference DNA circle reporting in a distinct fluorescence color.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.