ArticleNucleic acids research2002
Alternate exon insertion controls selective ubiquitination and degradation of different AUF1 protein isoforms.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 64 citations in OpenAlex.
- Gene expression and alternative splicing analysis in a large-scale Multiple Sclerosis study.medRxiv : the preprint server for health sciences · 2024Article
- HnRNP D activates production of HPV16 E1 and E6 mRNAs by promoting intron retention.Nucleic acids research · 2022Article
- PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM.Nucleic acids research · 2018Article
- Article
- The Regulation of Synaptic Protein Turnover.The Journal of biological chemistry · 2015Review
- Suppression of HPV-16 late L1 5'-splice site SD3632 by binding of hnRNP D proteins and hnRNP A2/B1 to upstream AUAGUA RNA motifs.Nucleic acids research · 2013Article
- Post-transcriptional regulatory networks in immunity.Immunological reviews · 2013Review
- Impairment of FOS mRNA stabilization following translation arrest in granulocytes from myelodysplastic syndrome patients.PloS one · 2013Article
- Concurrent binding and modifications of AUF1 and HuR mediate the pH-responsive stabilization of phosphoenolpyruvate carboxykinase mRNA in kidney cells.American journal of physiology. Renal physiology · 2012Article
- Involvement of the RNA-binding protein ARE/poly(U)-binding factor 1 (AUF1) in the cytotoxic effects of proinflammatory cytokines on pancreatic beta cells.Diabetologia · 2012Article
- Differential effects of hnRNP D/AUF1 isoforms on HIV-1 gene expression.Nucleic acids research · 2012Article
- AUF-1 and YB-1 are critical determinants of β-globin mRNA expression in erythroid cells.Blood · 2012Article
- Association of the von Hippel-Lindau protein with AUF1 and posttranscriptional regulation of VEGFA mRNA.Molecular cancer research : MCR · 2012Article
- Modulation of neoplastic gene regulatory pathways by the RNA-binding factor AUF1.Frontiers in bioscience (Landmark edition) · 2011Review
- Chaperone Hsp27 modulates AUF1 proteolysis and AU-rich element-mediated mRNA degradation.Molecular and cellular biology · 2011Article
- Members of the NuRD chromatin remodeling complex interact with AUF1 in developing cortical neurons.Cerebral cortex (New York, N.Y. : 1991) · 2008Article
- Proteasome inhibitor PS-341 (VELCADE) induces stabilization of the TRAIL receptor DR5 mRNA through the 3'-untranslated region.Molecular cancer therapeutics · 2008Article
- FRET-detectable interactions between the ARE binding proteins, HuR and p37AUF1.RNA (New York, N.Y.) · 2007Article
- Tumor necrosis factor activates a conserved innate antiviral response to hepatitis B virus that destabilizes nucleocapsids and reduces nuclear viral DNA.Journal of virology · 2007Article
- Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The A+U-rich element (ARE) in the 3' non-coding region (3' NCR) of short-lived cytokine mRNAs binds several regulatory proteins, including hnRNP D/AUF1, which comprises four isoforms of 37, 40, 42 and 45 kDa. ARE-mRNA degradation involves ubiquitin-proteasome activity, and one or more AUF1 proteins are thought to be ubiquitinated. Here we have characterized the mechanism for differential ubiquitination and degradation of the different AUF1 protein isoforms. We demonstrate in an in vitro ubiquitination system that the p37, followed by the p40 protein, are strongly ubiquitinated, whereas the p42 and p45 forms are not. Over expression in cells of enzymes that control the ubiquitin cycle were found to control p37 and p40 AUF1 protein levels through ubiquitination and proteasome activity, but not p42 and p45 forms. The p42 and p45 AUF1 proteins share a C-terminal exon 7 that is not found in the p37/p40 isoforms. Our studies show that exon 7 blocks ubiquitination and rapid degradation of AUF1 proteins, whereas its deletion permits ubiquitination to occur and promotes rapid turnover of AUF1 proteins. Thus, the stabilities of AUF1 isoforms are differentially controlled by insertion of an alternate exon that regulates ubiquitin targeting activity.
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