ArticleGenetics2002
Functional dissection of the global repressor Tup1 in yeast: dominant role of the C-terminal repression domain.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Tup1 ParalogmSphere · 2022Article
- Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.Current genetics · 2022Article
- Genetic analysis argues for a coactivator function for the Saccharomyces cerevisiae Tup1 corepressor.Genetics · 2021Article
- Article
- Functional analysis of the global repressor Tup1 for maltose metabolism in Saccharomyces cerevisiae: different roles of the functional domains.Microbial cell factories · 2017Article
- Article
- Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: the role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression.Microbial cell factories · 2016Article
- Effects of MIG1, TUP1 and SSN6 deletion on maltose metabolism and leavening ability of baker's yeast in lean dough.Microbial cell factories · 2014Article
- The Cyc8-Tup1 complex inhibits transcription primarily by masking the activation domain of the recruiting protein.Genes & development · 2011Article
- The general transcriptional repressor Tup1 is required for dimorphism and virulence in a fungal plant pathogen.PLoS pathogens · 2011Article
- Genome-wide analysis of the functions of a conserved surface on the corepressor Tup1.Molecular biology of the cell · 2005Article
- Functional comparison of the Tup11 and Tup12 transcriptional corepressors in fission yeast.Molecular and cellular biology · 2005Article
- DNA damage and replication stress induced transcription of RNR genes is dependent on the Ccr4-Not complex.Nucleic acids research · 2005Article
- Promoter-dependent roles for the Srb10 cyclin-dependent kinase and the Hda1 deacetylase in Tup1-mediated repression in Saccharomyces cerevisiae.Molecular biology of the cell · 2004Article
- Groucho oligomerization is required for repression in vivo.Molecular and cellular biology · 2004Article
- Membrane-coating lattice scaffolds in the nuclear pore and vesicle coats: commonalities, differences, challenges.Nucleus (Austin, Tex.)Review
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
In the yeast Saccharomyces cerevisiae, Tup1, in association with Cyc8 (Ssn6), functions as a general repressor of transcription. Tup1 and Cyc8 are required for repression of diverse families of genes coordinately controlled by glucose repression, mating type, and other mechanisms. This repression is mediated by recruitment of the Cyc8-Tup1 complex to target promoters by sequence-specific DNA-binding proteins. We created a library of XhoI linker insertions and internal in-frame deletion mutations within the TUP1 coding region. Insertion mutations outside of the WD domains were wild type, while insertions within the WD domains induced mutant phenotypes with differential effects on the target genes SUC2, MFA2, RNR2, and HEM13. Deletion mutations confirmed previous findings of two separate repression domains in the N and C termini. The cumulative data suggest that the C-terminal repression domain, located near the first WD repeat, plays the dominant role in repression. Although the N-terminal repression domain is sufficient for partial repression, deletion of this region does not compromise repression. Surprisingly, deletion of the majority of the histone-binding domain of Tup1 also does not significantly reduce repression. The N-terminal region containing potential alpha-helical coiled coils is required for Tup1 oligomerization and association with Cyc8. Association with Cyc8 is required for repression of SUC2, HEM13, and RNR2 but not MFA2 and STE2.
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