ArticleGenetics2002
Evidence that selected amplification of a bacterial lac frameshift allele stimulates Lac(+) reversion (adaptive mutation) with or without general hypermutability.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Selection and Plasmid Transfer Underlie Adaptive Mutation inGenetics · 2018Article
- Selection-Enhanced Mutagenesis ofGenetics · 2018Article
- Mutation--The Engine of Evolution: Studying Mutation and Its Role in the Evolution of Bacteria.Cold Spring Harbor perspectives in biology · 2015Review
- The Origin of Mutants Under Selection: How Natural Selection Mimics Mutagenesis (Adaptive Mutation).Cold Spring Harbor perspectives in biology · 2015Review
- Effect of growth under selection on appearance of chromosomal mutations in Salmonella enterica.Genetics · 2011Article
- The tandem inversion duplication in Salmonella enterica: selection drives unstable precursors to final mutation types.Genetics · 2010Article
- Mutability and importance of a hypermutable cell subpopulation that produces stress-induced mutants in Escherichia coli.PLoS genetics · 2008Article
- Amplification of lac cannot account for adaptive mutation to Lac+ in Escherichia coli.Journal of bacteriology · 2007Article
- Multiple pathways of selected gene amplification during adaptive mutation.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Article
- Adaptive mutation: how growth under selection stimulates Lac(+) reversion by increasing target copy number.Journal of bacteriology · 2004Article
- Rebuttal: adaptive point mutation (Rosenberg and Hastings).Journal of bacteriology · 2004Article
- Adaptive mutation in Escherichia coli.Journal of bacteriology · 2004Article
- Adaptive point mutation and adaptive amplification pathways in the Escherichia coli Lac system: stress responses producing genetic change.Journal of bacteriology · 2004Article
- Involvement of error-prone DNA polymerase IV in stationary-phase mutagenesis in Pseudomonas putida.Journal of bacteriology · 2004Article
- Adaptive mutation: general mutagenesis is not a programmed response to stress but results from rare coamplification of dinB with lac.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Error-prone polymerase, DNA polymerase IV, is responsible for transient hypermutation during adaptive mutation in Escherichia coli.Journal of bacteriology · 2003Article
- Formation of an F' plasmid by recombination between imperfectly repeated chromosomal Rep sequences: a closer look at an old friend (F'(128) pro lac).Journal of bacteriology · 2003Article
- Phenotypes of lexA mutations in Salmonella enterica: evidence for a lethal lexA null phenotype due to the Fels-2 prophage.Journal of bacteriology · 2002Article
- Stress-induced mutagenesis in bacteria.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
In the genetic system of Cairns and Foster, a nongrowing population of an E. coli lac frameshift mutant appears to specifically accumulate Lac(+) revertants when starved on medium including lactose (adaptive mutation). This behavior has been attributed to stress-induced general mutagenesis in a subpopulation of starved cells (the hypermutable state model). We have suggested that, on the contrary, stress has no direct effect on mutability but favors only growth of cells that amplify their leaky mutant lac region (the amplification mutagenesis model). Selection enhances reversion primarily by increasing the mutant lac copy number within each developing clone on the selection plate. The observed general mutagenesis is attributed to a side effect of growth with an amplification-induction of SOS by DNA fragments released from a tandem array of lac copies. Here we show that the S. enterica version of the Cairns system shows SOS-dependent general mutagenesis and behaves in every way like the original E. coli system. In both systems, lac revertants are mutagenized during selection. Eliminating the 35-fold increase in mutation rate reduces revertant number only 2- to 4-fold. This discrepancy is due to continued growth of amplification cells until some clones manage to revert without mutagenesis solely by increasing their lac copy number. Reversion in the absence of mutagenesis is still dependent on RecA function, as expected if it depends on lac amplification (a recombination-dependent process). These observations support the amplification mutagenesis model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.