ArticleMolecular biology of the cell2002
Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers.
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Who cites it
122 citing papers in PubMed, 234 citations in OpenAlex.
- A single-cell atlas of alternative wing development in two hemipteran species.Nature communications · 2026Article
- Somatic cells non-autonomously control germline incomplete cytokinesis through FGF signaling.bioRxiv : the preprint server for biology · 2026Article
- Semi-automated Detection of Late Cytokinetic Bridges.Methods in molecular biology (Clifton, N.J.) · 2026Article
- PP2A-B56 regulates Mid1 protein levels for proper cytokinesis in fission yeast.Molecular biology of the cell · 2025Article
- Cytokinetic abscission requires actin-dependent microtubule severing.Nature communications · 2024Article
- The Rho1 GTPase controls anillo-septin assembly to facilitate contractile ring closure during cytokinesis.iScience · 2023Article
- Article
- Localized efficacy of environmental RNAi in Tetranychus urticae.Scientific reports · 2022Article
- Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic apparatus.BMC biology · 2022Article
- Article
- TEM Imaging of Membrane Choreography During Mitosis of Drosophila Tissue Culture Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The ATPase SRCAP is associated with the mitotic apparatus, uncovering novel molecular aspects of Floating-Harbor syndrome.BMC biology · 2021Article
- Anillin propels myosin-independent constriction of actin rings.Nature communications · 2021Article
- Review
- Chromosomes function as a barrier to mitotic spindle bipolarity in polyploid cells.The Journal of cell biology · 2020Article
- Animal Cell Cytokinesis: The Rho-Dependent Actomyosin-Anilloseptin Contractile Ring as a Membrane Microdomain Gathering, Compressing, and Sorting Machine.Frontiers in cell and developmental biology · 2020Article
- The expression of four pyridoxal kinase (PDXK) human variants in Drosophila impacts on genome integrity.Scientific reports · 2019Article
- RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis.PLoS genetics · 2019Article
- Network Contractility During Cytokinesis-from Molecular to Global Views.Biomolecules · 2019Review
- A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism.BMC genetics · 2019Article
62 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used double-stranded RNA-mediated interference (RNAi) to study Drosophila cytokinesis. We show that double-stranded RNAs for anillin, acGAP, pavarotti, rho1, pebble, spaghetti squash, syntaxin1A, and twinstar all disrupt cytokinesis in S2 tissue culture cells, causing gene-specific phenotypes. Our phenotypic analyses identify genes required for different aspects of cytokinesis, such as central spindle formation, actin accumulation at the cell equator, contractile ring assembly or disassembly, and membrane behavior. Moreover, the cytological phenotypes elicited by RNAi reveal simultaneous disruption of multiple aspects of cytokinesis. These phenotypes suggest interactions between central spindle microtubules, the actin-based contractile ring, and the plasma membrane, and lead us to propose that the central spindle and the contractile ring are interdependent structures. Finally, our results indicate that RNAi in S2 cells is a highly efficient method to detect cytokinetic genes, and predict that genome-wide studies using this method will permit identification of the majority of genes involved in Drosophila mitotic cytokinesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.