ArticleMolecular biology of the cell2002
Contribution of Ena/VASP proteins to intracellular motility of listeria requires phosphorylation and proline-rich core but not F-actin binding or multimerization.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 101 citations in OpenAlex.
- LSP1-myosin1e bimolecular complex regulates focal adhesion dynamics and cell migration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion.eLife · 2020Article
- The VASP-profilin1 (Pfn1) interaction is critical for efficient cell migration and is regulated by cell-substrate adhesion in a PKA-dependent manner.The Journal of biological chemistry · 2019Article
- Article
- Hypoxia-induced up-regulation of VASP promotes invasiveness and metastasis of hepatocellular carcinoma.Theranostics · 2018Article
- A Noncanonical Binding Site in the EVH1 Domain of Vasodilator-Stimulated Phosphoprotein Regulates Its Interactions with the Proline Rich Region of Zyxin.Biochemistry · 2017Article
- Vasodilator-Stimulated Phosphoprotein Activity Is Required for Coxiella burnetii Growth in Human Macrophages.PLoS pathogens · 2016Article
- Threonine 89 Is an Important Residue of Profilin-1 That Is Phosphorylatable by Protein Kinase A.PloS one · 2016Article
- Crucial role for the LSP1-myosin1e bimolecular complex in the regulation of Fcγ receptor-driven phagocytosis.Molecular biology of the cell · 2015Article
- WAVE binds Ena/VASP for enhanced Arp2/3 complex-based actin assembly.Molecular biology of the cell · 2015Article
- S137 phosphorylation of profilin 1 is an important signaling event in breast cancer progression.PloS one · 2014Article
- Arp2/3-mediated actin-based motility: a tail of pathogen abuse.Cell host & microbe · 2013Review
- Defective FGF signaling causes coloboma formation and disrupts retinal neurogenesis.Cell research · 2013Article
- Endogenous cGMP-dependent protein kinase reverses EGF-induced MAPK/ERK signal transduction through phosphorylation of VASP at Ser239.Oncology letters · 2012Article
- Mena binds α5 integrin directly and modulates α5β1 function.The Journal of cell biology · 2012Article
- Fluorescence Resonance Energy Transfer (FRET)-based Detection of Profilin-VASP Interaction.Cellular and molecular bioengineering · 2011Article
- Molecular mechanism of Ena/VASP-mediated actin-filament elongation.The EMBO journal · 2011Article
- Metastasis: tumor cells becoming MENAcing.Trends in cell biology · 2011Review
- Defining a core set of actin cytoskeletal proteins critical for actin-based motility of Rickettsia.Cell host & microbe · 2010Article
- Differential VASP phosphorylation controls remodeling of the actin cytoskeleton.Journal of cell science · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The Listeria model system has been essential for the identification and characterization of key regulators of the actin cytoskeleton such as the Arp2/3 complex and Ena/vasodilator-stimulated phosphoprotein (VASP) proteins. Although the role of Ena/VASP proteins in Listeria motility has been extensively studied, little is known about the contributions of their domains and phosphorylation state to bacterial motility. To address these issues, we have generated a panel of Ena/VASP mutants and, upon expression in Ena/VASP-deficient cells, evaluated their contribution to Ena/VASP function in Listeria motility. The proline-rich region, the putative G-actin binding site, and the Ser/Thr phosphorylation of Ena/VASP proteins are all required for efficient Listeria motility. Surprisingly, the interaction of Ena/VASP proteins with F-actin and their potential ability to form multimers are both dispensable for their involvement in this process. Our data suggest that Ena/VASP proteins contribute to Listeria motility by regulating both the nucleation and elongation of actin filaments at the bacterial surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.