ArticleGenes & development2002
TFIID and human mediator coactivator complexes assemble cooperatively on promoter DNA.
Article in Genes & development, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Real-time visualization of reconstituted transcription reveals RNAPII activation mechanisms at single promoters.Cell reports · 2025Article
- Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3' ends.Cell reports · 2025Article
- Real-time visualization of reconstituted transcription reveals RNA polymerase II activation mechanisms at single promoters.bioRxiv : the preprint server for biology · 2025Article
- The Mediator complex as a master regulator of transcription by RNA polymerase II.Nature reviews. Molecular cell biology · 2022Review
- Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Spatiotemporal coordination of transcription preinitiation complex assembly in live cells.Molecular cell · 2021Article
- The Pol II preinitiation complex (PIC) influences Mediator binding but not promoter-enhancer looping.Genes & development · 2021Article
- Article
- Promoter-Enhancer Communication Occurs Primarily within Insulated Neighborhoods.Molecular cell · 2019Article
- Article
- The complex structure and function of Mediator.The Journal of biological chemistry · 2018Review
- Transcription regulation by the Mediator complex.Nature reviews. Molecular cell biology · 2018Review
- Genome-wide characterization of Mediator recruitment, function, and regulation.Transcription · 2017Review
- Transcriptional Regulation by Wild-Type and Cancer-Related Mutant Forms of p53.Cold Spring Harbor perspectives in medicine · 2017Review
- Toward understanding of the mechanisms of Mediator function in vivo: Focus on the preinitiation complex assembly.Transcription · 2017Review
- Mediator binding to UASs is broadly uncoupled from transcription and cooperative with TFIID recruitment to promoters.The EMBO journal · 2016Article
- Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture.Genes & development · 2016Article
- EP400 Deposits H3.3 into Promoters and Enhancers during Gene Activation.Molecular cell · 2016Article
- Article
- Mediator independently orchestrates multiple steps of preinitiation complex assembly in vivo.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Activator-mediated transcription complex assembly on templates lacking chromatin requires the interaction of activators with two major coactivator complexes: TFIID and mediator. Here we employed immobilized template assays to correlate transcriptional activation with mediator and TFIID recruitment. In reactions reconstituted with purified proteins, we found that activator, TFIID, and mediator engage in reciprocal cooperative interactions to form a complex on promoter DNA. Preassembly of the coactivator complex accelerates the rate of transcription in a cell-free system depleted of TFIID and mediator. Our data argue that this coactivator complex is an intermediate in the assembly of an active transcription complex. Furthermore, the reciprocity of the interactions demonstrates that the complex could in principle be nucleated with either TFIID or mediator, implying that alternative pathways could be utilized to generate diversity in the way activators function in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.