ArticleGenes & development2002
Fission yeast CENP-B homologs nucleate centromeric heterochromatin by promoting heterochromatin-specific histone tail modifications.
Article in Genes & development, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 114 citations in OpenAlex.
- PolySUMOylation of PCNA and Rad52 restricts centromeric recombination in fission yeast.Nature communications · 2025Article
- Revisiting the question: When is a centromere not a kinetochore?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- A systematic screen for co-option of transposable elements across the fungal kingdom.Mobile DNA · 2024Article
- Centromere innovations within a mouse species.Science advances · 2023Article
- The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.Nucleic acids research · 2022Article
- Evolution of eukaryotic centromeres by drive and suppression of selfish genetic elements.Seminars in cell & developmental biology · 2022Review
- Parallel pathways for recruiting effector proteins determine centromere drive and suppression.Cell · 2021Article
- Review
- The fission yeast CENP-B protein Abp1 prevents pervasive transcription of repetitive DNA elements.Biochimica et biophysica acta · 2016Article
- Evolutionary Turnover of Kinetochore Proteins: A Ship of Theseus?Trends in cell biology · 2016Review
- Article
- Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation.The Journal of biological chemistry · 2015Article
- Centromere sliding on a mammalian chromosome.Chromosoma · 2015Article
- Genetic and epigenetic regulation of centromeres: a look at HAC formation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Identification of novel α-n-methylation of CENP-B that regulates its binding to the centromeric DNA.Journal of proteome research · 2013Article
- γH2A-binding protein Brc1 affects centromere function in fission yeast.Molecular and cellular biology · 2013Article
- Epigenetic regulation of condensin-mediated genome organization during the cell cycle and upon DNA damage through histone H3 lysine 56 acetylation.Molecular cell · 2012Article
- CENP-B cooperates with Set1 in bidirectional transcriptional silencing and genome organization of retrotransposons.Molecular and cellular biology · 2012Article
- Article
- Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation.The Journal of cell biology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterochromatin is a functionally important chromosomal component, especially at centromeres. In fission yeast, conserved heterochromatin-specific modifications of the histone H3 tail, involving deacetylation of Lys 9 and Lys 14 and subsequent methylation of Lys 9, promote the recruitment of a heterochromatin protein, Swi6, a homolog of the Drosophila heterochromatin protein 1. However, the primary determinants of the positioning of heterochromatin are still unclear. The fission yeast proteins Abp1, Cbh1, and Cbh2 are homologs of the human protein CENP-B that bind to centromeric alpha-satellite DNA and associate with centromeric heterochromatin. We show that the CENP-B homologs are functionally redundant at centromeres, and that Abp1 binds specifically to centromeric heterochromatin. In the absence of Abp1 or Cbh1, the centromeric association of Swi6 is diminished, resulting in a decrease in silencing of the region. CENP-B-homolog double disruptants show a synergistic reduction of Swi6 at centromeric heterochromatin, indicating that the three proteins are functionally redundant in the recruitment of Swi6. Furthermore, using chromatin immunoprecipitation assays, we show that disruption of CENP-B homologs causes a decrease in heterochromatin-specific modifications of histone H3. These results indicate that the CENP-B homologs act as site-specific nucleation factors for the formation of centromeric heterochromatin by heterochromatin-specific modifications of histone tails.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.