ArticleAnnals of botany2002
Fluorescence in situ hybridization analysis of alien genes in Agrobacterium-mediated Cry1A(b)-transformed rice.
Article in Annals of botany, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Sequence analysis and potentials of the native RbcS promoter in the development of an alternative eukaryotic expression system using green Microalga Ankistrodesmus convolutus.International journal of molecular sciences · 2012Article
- In situ methods to localize transgenes and transcripts in interphase nuclei: a tool for transgenic plant research.Plant methods · 2006Article
- A novel approach for developing resistance in rice against phloem limited viruses by antagonizing the phloem feeding hemipteran vectors.Plant molecular biology · 2006Article
- Transgenic rice expressing Allium sativum leaf lectin with enhanced resistance against sap-sucking insect pests.Planta · 2006Article
- A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques.Plant cell reports · 2005Article
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Authors and funding
6 authors.
Funding
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Abstract
The transgene in Agrobacterium-mediated Cry1A(b)-transgenic rice plants has been detected and its chromosomal location determined by fluorescence in situ hybridization (FISH). Eight of the nine transgenic lines tested showed hybridization signals. Signals were located on regions of the chromosome in which fraction length (FL) values varied from 26.2 (near the centromere) to 95.2 (distal regions). No signal was found on regions where the fraction length was less than 26.2, while six of the nine signals detected were located on regions with FL values of 75.3 or over. This demonstrates that Agrobacterium-mediated genes can integrate into multiple sites distributed in different parts of the chromosome, but that distal regions are the preferred sites and regions near the centromeres are colder for T-DNA integration. The donor DNA of the transformation was divided into two parts, labelled separately as probes for two-colour FISH. Results show that the transformed DNA sequences remained linked in the recipient genome. The relationship between integration position and transgene silencing, known as the 'position effect', is discussed.
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