ArticleBiophysical journal2002
Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed, 164 citations in OpenAlex.
- Ghosts on the Membrane: Cytoskeletal Pinning Influences Nanoscale Cell Membrane Organization.Biomolecules · 2026Article
- Four-color fluorescence cross-correlation spectroscopy with one laser and one camera.Biomedical optics express · 2023Article
- Four-color fluorescence cross-correlation spectroscopy with one laser and one camera.bioRxiv : the preprint server for biology · 2023Article
- Alterations induced by the PML-RARα oncogene revealed by image cross correlation spectroscopy.Biophysical journal · 2022Article
- Single-vesicle intensity and colocalization fluorescence microscopy to study lipid vesicle fusion, fission, and lipid exchange.Frontiers in molecular neuroscience · 2022Article
- Nuclear envelope mechanobiology: linking the nuclear structure and function.Nucleus (Austin, Tex.) · 2021Review
- Degradation of Drug Delivery Nanocarriers and Payload Release: A Review of Physical Methods for Tracing Nanocarrier Biological Fate.Pharmaceutics · 2021Review
- Identifying Heteroprotein Complexes in the Nuclear Envelope.Biophysical journal · 2020Article
- Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins.Frontiers in molecular biosciences · 2019Review
- Impact of ConcanavalinA affinity in the intracellular fate of Protein Corona on Glucosamine Au nanoparticles.Scientific reports · 2018Article
- There and back again: from the origin of life to single molecules.European biophysics journal : EBJ · 2018Article
- Quantifying membrane protein oligomerization with fluorescence cross-correlation spectroscopy.Methods (San Diego, Calif.) · 2018Article
- Measuring Protein Binding to Lipid Vesicles by Fluorescence Cross-Correlation Spectroscopy.Biophysical journal · 2017Article
- Measuring protein dynamics in live cells: protocols and practical considerations for fluorescence fluctuation microscopy.Journal of biomedical optics · 2014Article
- Review
- Lysosome transport as a function of lysosome diameter.PloS one · 2014Article
- Photosystem II antenna phosphorylation-dependent protein diffusion determined by fluorescence correlation spectroscopy.Scientific reports · 2013Article
- Tracking image correlation: combining single-particle tracking and image correlation.Biophysical journal · 2013Article
- Shank2 contributes to the apical retention and intracellular redistribution of NaPiIIa in OK cells.American journal of physiology. Cell physiology · 2013Article
- Molecular brightness analysis reveals phosphatidylinositol 4-Kinase IIβ association with clathrin-coated vesicles in living cells.Biophysical journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence (auto)correlation spectroscopy (FCS) has developed into a widely used method for investigating molecular dynamics and mobility of molecules in vitro and in vivo. Dual-color cross-correlation, an extension of this technique, also assesses the concomitant movement of two spectrally distinguishable fluorescent molecules and has therefore proven superior to autocorrelation analysis to study interactions between different molecular species in solution. Here we explore the benefits of cross-correlation analysis when applied to live cells, by demonstrating its potential in analyzing endocytic processes. Bacterial cholera toxin (CTX) was labeled with Cy2 and Cy5 dyes on different subunits of the same holotoxin. Along the endocytic pathway, positive cross-correlation between the A and B subunits was first preserved, later followed by a loss in cross-correlation upon their separation in the Golgi. Furthermore, endocytosis of a mixture of only Cy2- and only Cy5-labeled holotoxins also gave rise to cross-correlation. Our results suggest that cross-correlation may be used to recognize whether different cargoes use the same endocytic pathway. Additionally, we show that cross-correlation is applicable to two-dimensional membrane diffusion. CTX bound to GM1-containing artificial giant unilamellar vesicles was diffusible, whereas CTX bound to the plasma membrane was immobile on the FCS time-scale, possibly because of raft-association of GM1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.