ArticleBiophysical journal2002
The role of Trp side chains in tuning single proton conduction through gramicidin channels.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Proton transfer reactions: From photochemistry to biochemistry and bioenergetics.BBA advances · 2023Article
- N-terminally glutamate-substituted analogue of gramicidin A as protonophore and selective mitochondrial uncoupler.PloS one · 2012Article
- Fluorescence techniques for determination of the membrane potentials in high throughput screening.Journal of fluorescence · 2010Review
- Gramicidin pores report the activity of membrane-active enzymes.Journal of the American Chemical Society · 2009Article
- The gramicidin channel ion permeation free-energy profile: direct and indirect effects of CHARMM force field improvements.Interdisciplinary sciences, computational life sciences · 2009Article
- Role of tryptophan residues in gramicidin channel organization and function.Biophysical journal · 2008Article
- Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy.Biophysical journal · 2006Article
- Proton transfer in gramicidin water wires in phospholipid bilayers: attenuation by phosphoethanolamine.Biophysical journal · 2006Article
- Proton conductance of influenza virus M2 protein in planar lipid bilayers.Biophysical journal · 2004Article
- Water alignment, dipolar interactions, and multiple proton occupancy during water-wire proton transport.Biophysical journal · 2004Article
- Proton transfer in gramicidin channels is modulated by the thickness of monoglyceride bilayers.Biophysical journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
We present an extensive set of measurements of proton conduction through gramicidin A (gA), B (gB), and M (gM) homodimer channels which have 4, 3, or 0 Trp residues at each end of the channel, respectively. In gA we find a shoulder separating two domains of conductance increasing with concentration, confirming the results of Eisenman, G., B. Enos, J. Hagglund, and J. Sandblom. 1980. Ann. NY. Acad. Sci. 339:8-20. In gB, the shoulder is shifted by approximately 1/2 pH unit to higher H(+) concentrations and is very sharply defined. No shoulder appears in the gM data, but an associated transition from sublinear to superlinear I-V values occurs at a 100-fold higher [H(+)] in gM than in gA. The data in the low concentration domain are analyzed using a configuration space model of single-proton conduction, assuming that the difference in the proton potential of mean force (PMF) between gA and its analogs is constant, similar to the results of Anderson, D., R. B. Shirts, T. A. Cross, and D. D. Busath. 2001. Biophys. J. 81:1255-1264. Our results suggest that the average amplitudes of the calculated proton PMFs are nearly correct, but that the water reorientation barrier calculated for gA by molecular dynamics using the PM6 water model (Pomès, R., and B. Roux. 1997. Biophys. J. 72:246a) must be reduced in amplitude by 1.5 kcal/mol or more, and is not rate-limiting for gA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.