ArticleBiophysical journal2002
Determination of cellular strains by combined atomic force microscopy and finite element modeling.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed.
- Measuring the Elastic Properties of Living Cells.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Improvement of Contact Models by Finite Element Analysis for the Evaluation of Yeast Mechanical Properties.Materials (Basel, Switzerland) · 2026Article
- The Diminishing Returns of Mechanical Loading and Potential Mechanisms that Desensitize Osteocytes.Current osteoporosis reports · 2021Review
- Stem Cell Mechanobiology and the Role of Biomaterials in Governing Mechanotransduction and Matrix Production for Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2020Review
- Anisotropy vs isotropy in living cell indentation with AFM.Scientific reports · 2019Article
- Probe Sensitivity to Cortical versus Intracellular Cytoskeletal Network Stiffness.Biophysical journal · 2019Article
- Mapping heterogeneity of cellular mechanics by multi-harmonic atomic force microscopy.Nature protocols · 2018Article
- Dehomogenized Elastic Properties of Heterogeneous Layered Materials in AFM Indentation Experiments.Biophysical journal · 2018Article
- Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology.Nature communications · 2017Article
- Review of cellular mechanotransduction on micropost substrates.Medical & biological engineering & computing · 2016Review
- Biomechanical assessment in models of glaucomatous optic neuropathy.Experimental eye research · 2015Review
- Altered mechanical environment of bone cells in an animal model of short- and long-term osteoporosis.Biophysical journal · 2015Article
- Mechanosensitivity of a rapid bioluminescence reporter system assessed by atomic force microscopy.Biophysical journal · 2015Article
- Cell morphology and focal adhesion location alters internal cell stress.Journal of the Royal Society, Interface · 2014Article
- Combining AFM and acoustic probes to reveal changes in the elastic stiffness tensor of living cells.Biophysical journal · 2014Article
- Nanobiomechanics of living cells: a review.Interface focus · 2014Review
- Mathematical modeling of the dynamic mechanical behavior of neighboring sarcomeres in actin stress fibers.Cellular and molecular bioengineering · 2014Article
- The effect of the endothelial cell cortex on atomic force microscopy measurements.Biophysical journal · 2013Article
- Mechanical properties of human amniotic fluid stem cells using nanoindentation.Journal of biomechanics · 2013Article
- A fluid-structure interaction model to characterize bone cell stimulation in parallel-plate flow chamber systems.Journal of the Royal Society, Interface · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many organs adapt to their mechanical environment as a result of physiological change or disease. Cells are both the detectors and effectors of this process. Though many studies have been performed in vitro to investigate the mechanisms of detection and adaptation to mechanical strains, the cellular strains remain unknown and results from different stimulation techniques cannot be compared. By combining experimental determination of cell profiles and elasticities by atomic force microscopy with finite element modeling and computational fluid dynamics, we report the cellular strain distributions exerted by common whole-cell straining techniques and from micromanipulation techniques, hence enabling their comparison. Using data from our own analyses and experiments performed by others, we examine the threshold of activation for different signal transduction processes and the strain components that they may detect. We show that modulating cell elasticity, by increasing the F-actin content of the cytoskeleton, or cellular Poisson ratio are good strategies to resist fluid shear or hydrostatic pressure. We report that stray fluid flow in some substrate-stretch systems elicits significant cellular strains. In conclusion, this technique shows promise in furthering our understanding of the interplay among mechanical forces, strain detection, gene expression, and cellular adaptation in physiology and disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.